一致地,在RKO和SW480细胞中过表达ALKBH3可以增加5’-tRF-GlyGCC的表达(图S4 C),而在HCT15和HCT116细胞中,抑制ALKBH3的表达可以降低5’-tRF-GlyGCC的表达(图S4 D)。证明了,tRNA去甲基化酶ALKBH3参与了CRC和血细胞中5’-tRF-GlyGCC的生物发生。 图4 ALKBH3参与了5’-tRF-GlyGCC的生物发生 5. 与CRC细胞...
Blood cells co-cultured with CRC cells or mice xenografted with CRC tumors show increased levels of 5′-tRF-GlyGCC. In addition, we find that the increased expression of 5′-tRF-GlyGCC is dependent on the upregulation of AlkB homolog 3 (ALKBH3), a tRNA demethylase which can promote ...
tRNA来源的小RNA(tRF)的生物学功能及在癌症中的作用
[16,19,20]. For example, Maute et al. used TargetScan to predict the targets of tRFGlyGCC, but only one in three was repressed by the tRF [16]. Similarly, Zhang et al. validated only one in five mRNAs predicted by miRanda and RNAhybrid using real-time PCR [21]. The accuracy of ...
(CRC) was significantly different from that of the healthy control group. This is particularly true for the expression of 5′-tRF and 5′-tRF-GlyGCC, whose levels are significantly increased in CRC plasma [43]. The clinicopathological data indicated that the levels of tRF-19-3L7L73JD ...
专利摘要:本发明涉及一种检测组织中tRF表达引物在制备甲基苯丙胺成瘾辅助诊断试剂盒的应用,其特征在于:该tRF‑1_32‑Gly‑GCC‑2‑M2的核苷酸序列如SEQIDNO:1所示,所述tRF‑1_32‑Gly‑GCC‑2‑M2在甲基苯丙胺成瘾大鼠组织中的特异性表达下调:所述引物为:F1:5’GATCGCATGGGTGGTTCAGT3';R1:5...
2022, Nucleic Acids Research Trnaglygcc-derived internal fragment (I-trf-glygcc) in ovarian cancer treatment outcome and progression 2022, Cancers View all citing articles on ScopusView full text © 2020 Elsevier Inc. All rights reserved. Part of special...
Arraystar公司推出的nrStar™ Human tRF & tiRNA PCR Array包含了185类来自权威数据库收录和最新文献报道的tRF或tiRNA,方便客户对tRF & tiRNA进行简单快捷地表达谱分析。
20x SSC buffer: 3 M sodium chloride, 300 mM sodium citrate. Prehybridization buffer: 0.75 M NaCl, 50 mM NaPO4, 5 mM EDTA, 1%SDS, 50% formamide, 0.2% BSA, 0.2% polyvinylpyrollidone 40, 0.2% Ficoll 400 (Sigma) trfRNAGlu—probe 5′ - CGCCGAATCCTAACCACTAGCCACCA ...
5003/4C, 5008C, 5009C, 5016C, 5026/27C, TRF62, TRF315, TRF327, TRF353, TRF419, tiRNA-5033-ProTGG-1, tiRNA-5033-GluTTC-1, tiRNA-5029-GlyGCC-2, tiRNA-5034-ValCAC-2, tiRNA-5034-ValCAC-3, tiRNA-5030-HisGTG-1, tiRNA-5030-GluTTC-1, tiRNA-5033-GluTTC-2, tiRNA-5030-LysCTT-...