测序结果显示,ESCC患者的外泌体中鉴定出1366个差异表达的 sesncRNA(不包括 miRNA)。挑选表达差异最显著的前五名sesncRNA进行表达检测验证,其中tRNA-GlyGCC-5和sRESE两者表达差异P值小于0.01。使用特异性的反义RNA进行转染,能显著抑制体外癌症细胞的多种恶性生物学行为。 图1 鉴定 ESCC 患者唾液外泌体中富含癌症的s...
One of these identical tRNA Gly GCC genes was found to be expressed at a substantially greater efficiency than the other in a HeLa cell lysate in vitro transcription system. No similarity was detected in the nucleotide sequences flanking these genes other than the characteristic, 3' oligo[dT] ...
Here, we report that inositol-requiring enzyme 1α (IRE1α) cleaves the anticodon stem-loop region of tRNAGly(GCC)to produce 5′-tRHs (5′-tRH-GlyGCC) with highly selective target discrimination upon endoplasmic reticulum (ER) stress. Levels of 5′-tRH-GlyGCCpositively affect cancer cell ...
5′-tRF-GlyGCCCRCALKBH3PlasmatRNA-derived small RNAsBackground tRNA-derived small RNAs (tDRs), which are widely distributed in human tissues including blood and urine, play an important role in the progression of cancer. However, the expression of tDRs in colorectal cancer (CRC) plasma and ...
In the two-dimensinal 1H-15N heteronuclear multiple-quantum correlation (HMQC) spectrum, only the peak at 12 ppm was observed, and thus it was assigned to the imino proton of the 5′-terminal G of GGCUp interacting with tRNAGCCGly. Judging from the temperature effect and chemical shifts, ...
Quigley F, Grienenberger JM, Weil JH: Localization and nucleotide sequences of the tRNA Gly (GCC), tRNA Asp (GUC) and tRNA Cys (GCA) genes from wheat chloroplasts. Plant Mol Biol 4:305–310, 1985.Quigley, F. R., J. M. Grienenberger and J. H. Weil (1985). Localization and ...