pTreg differentiationThis chapter shows protocols for the differentiation of peripheral Treg (pTreg) from polyclonal and monoclonal CD4doi:10.1007/978-1-0716-2647-4_6José.Almeida-SantosInstituto Gulbenkian de Ciência, Oeiras, Portugal. j.almeida-santos@imperial.ac.uk.Marie-Louise.Bergman...
在体外,初始的CD4+T辅助细胞受到IL-2和TGF-β诱导刺激后,可上调Foxp3转化为Treg细胞。 昨天,站内有站友问Treg扩增的详细步骤(http://www.flowcyto.cn/bbs/thread-7807-1-1.html),于是我们找到了一篇较为详细的Protocol《[Mouse N...
在体外,初始的CD4+T辅助细胞受到IL-2和TGF-β诱导刺激后,可上调Foxp3转化为Treg细胞。 昨天,站内有站友问Treg扩增的详细步骤(http://www.flowcyto.cn/bbs/thread-7807-1-1.html),于是我们找到了一篇较为详细的Protocol《[Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Sub...
细胞凋亡、细胞黏着、细胞周期等,不仅有细胞培养相关实验,还有包括不同研究水平实验技术Protocol,不同实验方法全流程,WB实验流程、注意事项、数据处理及写作、IHC实验流程、操作技巧、注意事项、图像分析等相关实验的详细步骤,全都是经过前辈们无数次验证过的,希望对大家的实验有帮助。
Figure 3. ImmunoCult™ Human Treg Differentiation Supplement Produces FOXP3+ Cells Under Treg Polarizing Conditions Naïve CD4+ T cells were cultured for 7 days in ImmunoCult™ activator, supplement and medium using the protocol described in Figure 2. Cells were stained for CD4, CD4, CD25...
Regulatory T-cells (Tregs) are central in the maintenance of homeostasis and resolution of inflammation. However, the mechanisms that govern their differentiation and function are not completely understood. Herein, we demonstrate a central role for the lipid mediator biosynthetic enzyme 15-lipoxygenase ...
It has been shown that 9-cis-retinoic acid (9cRA) blocks ex vivo apoptosis of peripheral blood lymphocytes and promotes the differentiation of Treg cells in the gut. However, the role of retinoid signaling in regulating the immune privilege of the testes remains unknown. Objective The aim of...
Fig. 1: NOR promotes Treg differentiation under hypoxic and normoxic microenvironment. aCD4+T cells were cultured with NOR (0.1, 0.3, 1, 3, 10, 30, 60, 100 μM) in hypoxia or normoxia for 72 h and cell viability was analyzed by MTT and CCK-8.bCD4+T cells were cultured with ...
(+) cells from the cultures enriched for cells with regulatory activity; as now 100% survival of C57BL/10 skin grafts was achieved. Furthermore, differentiation of Treg could be also achieved when using purified CD25(-)CD4(+) naive T cells as a starting population. Interestingly, further in...
Expression levels of the transcription factor Foxp3 have been causally associated with Treg differentiation and function. Recent studies show that Foxp3 can also be transiently expressed in effector T cells; however, stable Foxp3 expression is required for development of a functional Treg suppressor ...