ImmunoCult™ Human Treg Differentiation Supplement ImmunoCult™ Human Treg Differentiation Supplement contains a human cytokine and small molecule formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived
Human Treg Reagent 2 Human Treg Reagent 3 Human Treg Reagent 4 Reconstitution Buffer 1 Reconstitution Buffer 2 Wash Buffer (20X) Provides sufficient reagents for the differentiation of one 24-well plate. Stability and Storage Store the unopened kit at 2-8°C. After opening the kit, the Mouse...
To gain a comprehensive and unbiased molecular understanding of FOXP3 induction, we performed time-series RNA sequencing (RNA-Seq) and proteomics profiling on the same samples during human iTreg differentiation. To enable the broad analysis of universal FOXP3-inducing pathways, we used five ...
Th2 differentiation of converted FOXP3(-) ex-Treg occurred even under nonpolarizing conditions and could not be prevented by IL-4 signaling blockade. Thus, our studies identify Th2 differentiation as the default developmental program of human Treg after downregulation of FOXP3. 展开 ...
Protocol Technical Advice It is generally recommended to use a bead-to-cell ratio of 4:1 and the cell concentrations given in Table 1. Other bead-to-cell ratios and cell concentrations can be used following optimization fo...
CellXVivo Human Treg Cell Differentiation Kit (1 KT) 价格: ￥4490.00 ￥4490.0 评论:0条 收藏:0 产品详情 产品评论(0) 销售记录(0) 品牌:R&D systems型号:CDK006 规格:1 KT
Human FOXP3+ regulatory T (Treg) cells are central to immune tolerance. However, their heterogeneity and differentiation remain incompletely understood. Here we use single-cell RNA and T cell receptor sequencing to resolve Treg cells from healthy individ
These CD39+ γδTreg cells suppress the activity of human CD3+ T cells in an adenosine-dependent manner (unpublished data). Il-17-producing γδt cells γδT17 cells broadly participate in inflammatory responses, having pathogenic roles during infection and autoimmune diseases.74 Differentiation ...
The protocol for Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Cat. No. 11131D) is optimized for the activation of naive T cells, whereas the protocol for Dynabeads Human Treg Expander (Cat. No. 11129D) is optimized for regulatory T cell expansion. Both products...
To evaluate the epithelial cell states with respect to their order in the differentiation trajectory, we conducted pseudotime analysis on all epithelial and tumor cells identified in the PCa samples. We first calculated a PAGA (partition-based graph abstraction) graph using SCANPY’s sc.tl.paga()...