(AR)患者和小鼠中的表达和意义.方法 收集6例对照组患者(Control组)和6 例变应性鼻炎患者(AR 组)的鼻黏膜,检测其鼻黏膜炎症反应.同期收集 12 例 Control 组和 12 例 AR 组患者外周血单个核细胞 (PBMC),检测其 Treg 细胞和 GATA3+Treg 细胞比例.将 C57BL/6 小鼠分为正常对照组(Control 组)和变应性...
RegulatoryTcellsFOXP3TGF-13ImmunetoleranceW 天津医科大学硕士学位论文CDCFSEDEPCDMSOELISAFBSFCMFITCFOXP3ILMACSMOIPBMCPBSPERT-PCRTGF.13Treg缩略语Clusterofdifferentiation分化群Carboxyfluoresceinsuccinimidylester羧基荧光素琥珀酰亚胺酯DiethylpyrocarbonatediInethylsulfoxide二乙基焦磷酰胺二甲基亚砜Enxyme—linked...
Human FOXP3+regulatory T (Treg) cells are central to immune tolerance. However, their heterogeneity and differentiation remain incompletely understood. Here we use single-cell RNA and T cell receptor sequencing to resolve Tregcells from healthy individuals and patients with or without acute graft-vers...
Regulatory T cells (Treg) play a key role in maintaining the balance between Th1 and Th17 immune responses, while interleukin-27 (IL-27) levels are related to the differentiation of Th17 cells. This study analyzes the differences in the number of Treg cells and the level of I...
Regulatory T-cells (Tregs) are central in the maintenance of homeostasis and resolution of inflammation. However, the mechanisms that govern their differentiation and function are not completely understood. Herein, we demonstrate a central role for the l
调节性T细胞(Regulatory cells,Tregs ),早期亦称为Suppressor T cells,是一群以表达Foxp3、CD25、CD4为细胞表型特征的T细胞亚群,是维持机体免疫耐受的重要因素之一。胸腺是Treg细胞发育的主要部位。 图片来源:Foxp3-dependent programme of regulatory T-cell differentiation ...
结合 本研究结果推测,LN 患者体内 Treg 细胞比例的下降, 可能诱导 PBMC 中 ROR⁃γt 调控功能增强,增加 Th17 细胞炎性反应效应及其对下游炎性细胞因子网络的调 控作用. 本研究中 LN 患者 Treg 细胞比例同 Foxp3 mRNA 表达水平,以及 Th17 细胞比例同 ROR⁃γt mR⁃ NA 表达水平并未表现出完全一致的...
Regulatory T cells (Tregs) are crucial in maintaining immune homeostasis and preventing autoimmunity and inflammation. A proportion of Treg cells can lose Foxp3 expression and become unstable under inflammation conditions. The precise mechanisms underlyi
在PBMC中分别标记CD4、CD25、CD127抗体,避光孵育20 min后,磷酸盐缓冲液(PBS)清洗,离心弃上清,重悬细胞,上流式仪器检测Treg细胞比例。同时均设置同型对照组。 四、统计学处理方法 采用SPSS 22.0软件分析,外周血Th17细胞、Treg细胞比例为计量资料,方差齐性检验后,正态分布数据以± s表示;四组研究对象外周血Th17细胞...
Targeting glucocorticoid-induced TNFR-related receptor (GITR) in Treg cells using an agonistic antibody (αGITR) promotes CD4 Treg cell differentiation into CD4 effector T cells, alleviates Treg cell-mediated suppression of anti-tumor immune response, and induces potent anti-tumor effector cells in ...