which is a sensitive live-cell imaging method to measure the interaction of CSB with lesion-stalled Pol II46. CSB–mScarlet-I FRAP showed strong and dose-dependent immobilization after FA treatment, with 300 µM of FA resulting in a similar CSB immobilization level as ...
Centromeres are established by nucleosomes containing the histone H3 variant CENP-A. CENP-A is recruited to centromeres by the Mis18–HJURP machinery. During mitosis, CENP-A recruitment ceases, implying the necessity of CENP-A maintenance at centromeres,
Summary Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within transcriptional networks. Here, we present a synthetic framework for systematically constructing eukaryotic transcription functions using artificial zinc fingers, modular DNA-binding domains found within many eukaryotic...
With the advent of modern molecular biology, measuring mRNA levels in cells became a regular test to address whether genes are regulated at the transcriptional level. For genes involved in the cell cycle, understanding gene regulation at the transcriptional level requires measuring mRNA abundance over ...
Summary Intestinal homeostasis is tightly regulated by complex yet poorly understood signaling networks. Here, we demonstrate that Lats1/2, the core Hippo kinases, are essential to maintain Wnt pathway activity and intestinal stem cells. Lats1/2 deletion leads to loss of intestinal stem cells but ...
The results presented here might also impact synthetic biology efforts to engineer theS. cerevisiaegenome. In a pilot study that reported the construction of a minimal yeast chromosome III, eleven tDNAs were deleted without any significant effect on fitness [50]. Inspection of the IGV transcriptome...
SummaryIn order to assess specific functional roles of plant heat shock transcription factors (HSF) we conducted a transcriptome analysis of Arabidopsis th... Wolfgang,Busch,Markus,... - 《Plant Journal》 被引量: 684发表: 2004年 Cooperative binding of Drosophila heat shock factor to arrays of ...
adN/dSestimate of 0.1567 was obtained, which showed that the soybean MYB domain is under strong negative selection (neutral selection corresponds to a ratio of 1). At the individual codon level, we found 99 residues were under negative selection in MYB genes without a single positively selected...
e Summary of induction and modulation of transient YAP localization-resets through LATS kinase. f Proposed model for the relationship between YAP dynamics and YAP responsive transcription. Dashed line represents N/C = 1. Full size image Perhaps, the chronically elevated transcriptional regulation ...
Genes with generally important roles in CNCC biology but causing phenotypes distinct from those associated with SOX9 are buffered against SOX9 dosage change, but a subset of sensitive genes impacts specific cellular processes and morphological features similar to those associated with SOX9. Specifically, ...