Moreover, Aβ1−42 raises AP-1 DNA binding activity in THP-1-treated cells. Interestingly, all these effects were reduced in the presence of BJe. Our data indicate that BJe may effectively counteract the pro-inflammatory activation of monocytes/microglial cells exposed to amyloid fibrils, ...
通过研究发现，咪达唑仑可通过激活TSPO（转录启动子复合体）信号传导，抑制LPS（脂多糖）诱导的THP-1巨噬细胞活化，从而减少促炎因子的表达，影响NF-kB/AP-1的激活。这些研究不仅揭示了苯二氮卓类药物的免疫调节作用及其分子机制，也为理解其在危重患者中可能产生的副作用提供了科学依据。构建THP-1稳转...
同时观察当NF-κB,AP-1和MARK信号通路抑制剂存在时,CRP对LOX-1抗原和mRNA表达的影响.结果 CRP促进巨噬细胞LOX-1蛋白和mRNA表达增加.NF-κB的抑制剂BAY11-7085能抑制CRP对LOX-1蛋白和mRNA表达的诱导作用.结论 CRP能在转录及转录后水平诱导THP-1来源的巨噬细胞表达LOX-1,这种调节作用可能是通过NF-κB信号传导...
TSPO配体降低了LPS诱导的THP-1 Xblue CD14细胞中NF-kB/AP-1的激活。这些结果表明咪达唑仑通过激活TSPO信号传导降低促炎因子的表达,进而阻断NF-kB/AP-1对相关促炎基因的反激活。 为了进一步研究咪达唑仑对LPS处理的THP-1细胞的抑制作用是否依赖于TSPO信号,作者构建了TSPO过表达的THP-1稳转细胞系,比较咪达唑仑对...
Once activated, both TLR7 and TLR8 recruit the adaptor protein MyD88 to trigger IRF, AP-1, and NF-κB responses via TRAF6 (TNF receptor-associated factor 6) [1,3]. Depending on the stimulus and cell type, TLR7-mediated signaling induces IFN-α and IFN-regulated cytokines or T helper ...
It has a higher saturation threshold than with pNPP (p-nitrophenyl phosphate) resulting in more significant differences between no, low or high AP activity. Another advantage of QUANTI-Blue™ is that it can determine secreted AP activity without disturbing cells, thus allowing the repeated ...
TLR4 signaling activates the pro-inflammatory transcription factor AP-1 through ERK-dependent phosphorylation22. To confirm the role of the ERK signal pathway in the anti-inflammatory mechanisms of OEA, THP-1 cells were subjected to PD098059 (1 μM) for 30 min followed by treatment with OE...
Importantly, OEA inhibited LPS-induced NF-κB activation, IκBα degradation, expression of AP-1, and the phosphorylation of ERK1/2 and STAT3. In summary, our results demonstrated that OEA exerts anti-inflammatory effects by enhancing PPARα signaling, inhibiting the TLR4-mediated NF-κB ...
receptors, TLR1 or TLR6, which is crucial for signaling and ligand specificity. TheTLR2/TLR1andTLR2/TLR6heterodimers specifically bind lipoproteins depending on whether they aretri- or diacylated, respectively [1]. Their activation triggers NF-κB- and AP-1-mediated pro-inflammatory responses ...
THP-1巨噬细胞热休克处理对单核细胞超化蛋白-1和白细胞介素表达的影响