However, all three chosen protocols which use Vit D3, IL-13/IL-4, and IL-4, respectively, failed to promote the polarization of macrophages with a reliable M2 phenotype. Therefore, optimization or development of a new M2 polarization protocol is needed to achieve macro...
(2010) published a protocol for THP-1 cell differentiation, showing that THP-1 cells represent a simplified model to study monocyte-macrophage polarization (Qin 2012). U937 is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old ...
THP-1 is an immortalized cell line that can be cultured in vitro up to passage 25 (approx. 3 months) without changes of cell sensitivity and activity. As far as our information reaches, U937 is used also at higher passage numbers (see e.g. Strefford et al.2001). THP-1 as well as ...
THP-1 cell-line presents a simplified, suitable and reliable model to mimic the monocyte/macrophage polarization, differentiation, functions, possible effects from external stimuli and responses of the surrounding environment. THP-1 is broadly used to learn monocyte/macrophagesignalling pathways, mechanisms...
a cytokine profile that resembled MDM in response to TLR ligands,in particular with marked TLR2responses.Moreover,both MDM and PMAr retained marked plasticity to stimulus-directed polarization.These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1...
比较不同诱导条件下的细胞THP-1细胞
Authors in their studies show different approaches using either induced to differentiation or undifferentiated cells as neurobiological models, often without considering this discrepancy in cell lines preparation protocol. Our study investigated the impact of PC12 or THP-1 cell differentiation on sensitivity...
de Korne, C.M.; Chaye, M.A.M.; Gasan, T.A.; et al. Fasciola hepatica Fatty Acid Binding Protein 1 Modulates T cell Polarization by Promoting Dendritic Cell Thrombospondin-1 Secretion Without Affecting Metabolic Homeostasis in Obese Mice.Front. Immunol.2022,13, 884663. [Google Scholar] [...
followed by detachment of the cells and eletroporation using Lonza nucleofector technology andfinally includes further 48 h of differentiation with 100 ng/ml PMA. When we applied this protocolto study interleukin (IL) 10 dependent polarization, the cells were inert to the IL10 stimulus, as...
THP-1 用185ng/ml佛波酯(PMA,用DMSO溶解)作用6小时,诱导细胞分化为巨噬细胞。然后,在PMA继续存在的情况下,通过分别: •与IFN-γ(20 ng/ml)和LPS(100 ng/ml)孵育48小时以上,使其向M1极化。•与IL-4(20 ng/ml)和IL-13(20 ng/ml)孵育48小时以上,使其向M2表型极化。