mRNA stability, and translation10,11. Notably, hnRNPs have also been found to directly or indirectly influence miRNA expression by modulating pri- and/or pre-miRNA processing12. Furthermore, hnRNPs can indirectly compete with or inhibit the miRNA-induced ...
We have studied this phenomenon in an effort to gain more insight into the degradation pathway of this mRNA. Our results indicate that the stabilization of c-myc mRNA in the absence of translation can be fully explained by the inhibition of translation-dependent poly(A) tail shortening. This ...
Regulation of poly(A) tail length during mRNA 3'-end formation requires a specific poly(A)-binding protein in addition to the cleavage/polyadenylation machinery. The mechanism that controls polyadenylation in mammals is well understood and involves the nuclear poly(A)-binding protein PABPN1. In co...
Moreover, findings revealed a mechanism of mRNA silencing through deadenylation that involves the recruitment of the deadenylase CAF1 and poly(A)-binding protein (PABP) to the RNA-induced silencing complex (RISK).doi:10.1038/nrm2781David
Following the second-strand cDNA synthesis by DNA polymerase I, the cDNA fragments were purified using a QiaQuick PCR extraction kit (Qiagen, Venlo, The Netherlands). After a poly (A) tail was added, the resulting fragments were ligated to Illumina sequencing adapters. The ligation products ...
aThe second modification of eukaryotic mRNA is adding a long stretch of adenines to the 3´end –the poly(A) tail. eukaryotic mRNA的第二修改增加长的舒展adenines到3´end -多(A)尾巴。[translate]
identified by the A-seq method in a human cell line ► Binding sites of pre-mRNA 3′ end processing factors are mapped by PAR-CLIP ► CstF-64 and CF Im68 exhibit the highest positional binding specificity ► CF Im 68 siRNA treatment causes a global shift toward proximal poly(A) ...
To determine that the translation of the His-tagged proinsulin-expressing mRNAs mirrored the endogenous preproinsulin mRNA as closely as possible, the termini were mapped. A, primer extension performed upon poly(A)+ from the β-cell line, INS-1 (I), or primary hepatocytes (H), either uninfe...
mRNA or DNA sequence, nucleic acid vaccines can bypass the need for HLA matching. The produced antigen is processed by the recipient's cells, leading to the presentation of peptides on the cell surface in a manner that is independent of the individual's HLA type [8]. This ability to ...
An evolutionarily conserved mRNA-degradation pathway is initiated by the removal of the 3′ poly(A) tail. This disrupts the translation initiation complex and provides degradative enzymes with access to the 5′ cap and remaining RNA body.