However, unlike eukaryotic cells, mRNA sequencing of bacterial samples is more challenging due to the absence of a poly-A tail that typically enables efficient capture and enrichment of mRNA from the abundant rRNA molecules in a cell. Moreover, bacterial cells frequently contain 100-fold lower ...
while also highlighting the necessity of single-cell resolution (Supplementary Fig.8D–E). For ourS. aureussamples, BaSSSh-seq captured an average of 34 and 60 mRNA reads per cell during biofilm and planktonic growth, respectively (a range of 7 to >2000 reads per cell and 1 to >1000 dete...
transcripts in contrast to the more abundant (plant) transcripts, along with the substantial difference in total RNA content where the host significantly outnumbers the bacterial counterpart [15]. Therefore, along with the removal of rRNA, it is imperative to implement mRNA enrichment approaches to ...
The collective sequence consisted of a 1,992-bp open reading frame (ORF) and a 1,168-bp 3′-UTR. Three polyadenylation signals were found in the 3′-UTR, the last of which was followed by the poly(A) tail. The predicted amino acid sequence of this cDNA is 664 amino acids (Fig. ...
Information beyond mRNA expression profiles and pattern recognition spectrum are needed to determine the exact roles of these LRR-only proteins in scallop innate immunity. In the present study, a novel LRR-only protein from C. farreri (designated CfLRRop-7) has been identified and investigated. ...
(e) Animals exposed to empty vector (EV) or pink-1(RNAi) were assessed for lgg-1 mRNA levels with or without S. ven metabolite treatment. This treatment occurred until the L4 stage. Three replicates comprises at least 100 animals each; 1 μg of RNA was subjected to cDNA synthesis. ...
Gene expression is regulated at both transcriptional and posttranscriptional levels. Foreign genetic elements have evolved to integrate their expression systems into the host regulatory network. Transcription of AT-rich foreign genes is globally silenced
(Fig.2D-F). A significant reduction in the abundance ofc-MycmRNA was confirmed at 3 time points (24 h, 48 and 72 h) post-infection due to bacterial delivery ofSL7207/c-Myccompared to aSL7207/SCR(Fig.2G-I). RNAi was accompanied by a corresponding reduction of the protein, although ...
The results (Lane 16) showed that RelE cleaved the mRNA with intermediate efficiency in the first (UUU) and second (ACG) codon after AUG. The translation factors that were used to construct the RC contained an exonuclease activity that removed the heterogeneous polyA tail of the XR7 mRNAs ...
The cDNA of SoRab1 contains a 5′-UTR of 358 bp, an ORF of 612 bp, and a 3′-UTR of 265 bp (Fig. 1). The putative polyadenylation sequence, AATAAA, is located 18 bp upstream the poly(A) tail. The ORF encodes a putative protein of 203 residues with a predicted molecular mass ...