T cells that recognize HLA-E peptides via their T cell receptor (TCR) are termed donor-unrestricted T cells due to restricted allelic variation of HLA-E. The composition and repertoire of HLA-E TCRs is not known so far. We performed TCR sequencing on CD8 + T cells from 21 individuals ...
CD8 is known to bind HLA to assist in TCR and HLA binding. This means that HLA contains a CD8-binding site. Therefore, it occasionally causes false positive signals between HLA tetramers and CD8-positive T cells. *Please note that α3 mutation is adopted only in Human HLA Class I Tetram...
Epitope specificity of clonally expanded populations of CD8+ T cells found within the joints of patients with inflammatory arthritis. The TCR repertoire of the virus-specific T cells in the synovial fluid was analyzed using a panel of TCR β variable region–specific monoclonal ... C Fazou,H ...
Persistent numbers of tetramer+ CD8(+) T cells, but loss of interferon-gamma+ HIV-specific T cells during progression to AIDS. 2002. Persistent numbers of tetramer+ CD8(+) T cells, but loss of interferon-gamma+ HIV-specific T cells during progression to AIDS. Blood 99:2505-... Stefan,...
These populations are a unique source of cells for cytotoxicity assays [15,16,33] and TCR repertoire analyses [37]. It also appears that the fluorescence intensity of tetramer staining on antigen-specific CD8+ T cells correlates with their avidity for the antigen [38,39]. Hence, tetramer ...
any peptide. ProT2® Class II MHC Tetramers are delivered quickly with stringent quality control and low batch-to-batch variability. As such ProT2® Class II MHC Tetramers complement ProImmune’s existing Pro5® MHC Class I Pentamer technology for detecting antigen-specific CD8+ T cells....
Tetramer-positive cells are antigen specific, but not uniformly antigen responsive. Tumour-specific CD8+ T-cell responses do arise spontaneously, but differ from antiviral responses in magnitude and, potentially, duration. Spontaneous tumour-specific CD8+ T-cell responses might often be 'too little...
Human CD8+ T-cells Recognizing Peptides from Mycobacterium tuberculosis (Mtb) Presented by HLA-E Have an Unorthodox Th2-like, Multifunctional, Mtb Inhibito... Mycobacterial antigens are not exclusively presented to T-cells by classical HLA-class Ia and HLA-class II molecules, but also through ...
Functions of tetramer-stained HIV-specific CD4(+) and CD8(+) T cells. Curr. Opin. Immunol. 12, 370-374.A.D. Kelleher, S.L. Rowland-Jones, ... AD Kelleher,SL Rowland-Jones - 《Current Opinion in Immunology》 被引量: 60发表: 2000年 An in vivo cytotoxicity threshold for influenza A...
Selective deletion of antigen-specific CD8+ T cells by MHC class I tetramers coupled to the type I ribosome- inactivating protein saporin. Blood. 2007;109(8):3300-7.P. Hess, C. Barnes, M. Woolard et al., "Selective deletion of antigen-specific CD8+ T cells by MHC class I tetramer...