如抗原特异性细胞毒性T细胞(CTL)主要识别的是MHC I类分子的内源性抗原肽,CTL通过TCR识别并结合递呈在靶细胞表面的MHC I-多肽复合物结合槽内的特异性抗原表位,在共同刺激信号的协助下活化、增生形成表位特异性的T细胞克隆进而发挥生理效应。
经流式细胞水平验证,ACROBiosystems 自主开发的HLA-A*02:01&B2M&NY-ESO-1Tetramer产品,可很好的与TCR结合,且活性明显高于竞品 2e5 of NY-ESO-1 TCR-HEK293 cells (pool) were stained with PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) tetramer Pr...
经流式细胞水平验证,ACROBiosystems 自主开发的HLA-A*02:01&B2M&NY-ESO-1Tetramer产品,可很好的与TCR结合,且活性明显高于竞品 2e5 of NY-ESO-1 TCR-HEK293 cells (pool) were stained with PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) tetramer Protein(Fig B)and negative control protei...
Tetramers for CD4+T cells and natural killer T cells exist but have yet to make the same impact as MHC class I tetramers. However, tetramers of non-classical MHC class I molecules (for example, HLA-E) have been important in identifying new pathways of natural-killer-cell regulation. The...
MHC四聚体检测技术是在单细胞水平上标记目标T细胞,并通过流式细胞术对细胞进行分析,从而成为一种快速、简便的检测抗原特异性T细胞(antigen-specificT cells)的定性及定量分析方法。 TCR与MHC-多肽复合物 MHC分子在免疫应答过程中发挥着抗原识别的作用,主要功能是将抗原呈递给T细胞,以区分自身(我们的细胞和组织)和非己...
Klenerman P, Cerundolo V, Dunbar PR (2002) Tracking T cells with tetramers: new tales from new tools. Nat Rev Immunol 2:263-272Klenerman P, Cerundolo V, Dunbar PR (2002) Tracking T cells with tetramers: new tales from new tools. Nat Rev Immunol 2: 263-272....
MHC 四聚体检测技术于 1996 年由美国斯坦福大学医学院的 John D. Altman 博士所开发, 它在单细胞水平上标记目标 T 细胞,并通过流式细胞术对细胞进行分析,从而成为一种快速、简便的检测抗原特异性 T 细胞(antigen-specific T cells)的定性及定量分析方法。
Tetrameric peptide–MHC-class-I complexes (tetramers) allow antigen-specific T cells to be tracked in time and space, as well as a detailed analysis of their surface phenotype. Tetramers are best used in parallel with functional assays. Very large, focused expansions of T-cell populations ...
关键词 人乳头瘤病毒 ;四聚体 ; T淋巴细胞中图分类号 R373 文献标识码 A 文章编号 1005 - 7021(2010) 04 - 0017 - 05Opti m ization of Tetramer Sta in ing Condition to Detectof Human Papillomavirus Specific T CellsWANG Xue2lian(1. Teach & Res . Div . of Pathog. B iol . , China M ...
Tetramer-visualized gluten- specific CD4+ T cells in blood as a potential diagnostic marker for coeliac disease without oral gluten challenge. United Eur Gastroenterol J 2014;2:268-78.Christophersen, A., M. Ra´ki, E. Bergseng, K. E. Lundin, J. Jahnsen, L. M. Sollid, and S. W....