经流式细胞水平验证,ACROBiosystems 自主开发的HLA-A*02:01&B2M&NY-ESO-1Tetramer产品,可很好的与TCR结合,且活性明显高于竞品 2e5 of NY-ESO-1 TCR-HEK293 cells (pool) were stained with PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) tetramer Pr...
如抗原特异性细胞毒性T细胞(CTL)主要识别的是MHC I类分子的内源性抗原肽,CTL通过TCR识别并结合递呈在靶细胞表面的MHC I-多肽复合物结合槽内的特异性抗原表位,在共同刺激信号的协助下活化、增生形成表位特异性的T细胞克隆进而发挥生理效应。
经流式细胞水平验证,ACROBiosystems 自主开发的HLA-A*02:01&B2M&NY-ESO-1Tetramer产品,可很好的与TCR结合,且活性明显高于竞品 2e5 of NY-ESO-1 TCR-HEK293 cells (pool) were stained with PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) tetramer Protein(Fig B)and negative control protei...
Traditional functional assays for studying antigen-specific CD4+ T cells, such as proliferation or cytokine secretion, are not truly antigen-specific. The direct detection of antigen-specific CD4+ T cells has been difficult, due to the low frequency of these T cells (down to 1 in 100,000 whi...
检测技术是在单细胞水平上标记目标T细胞,并通过流式细胞术对细胞进行分析,从而成为一种快速、简便的检测抗原特异性T细胞(antigen-specificT cells)的定性及定量分析方法。 MHC分子在免疫应答过程中发挥着抗原识别的作用,主要功能是将抗原呈递给T细胞,以区分自身(我们的细胞和组织)和非己(入侵者或修饰的自身)。以人类为...
MHC tetramers are used for the detection and monitoring of antigen-specific T cells to study the adaptive immune response, including T cell development, disease progression, and evaluation of therapeutics, vaccinations, and other interventions that may have an impact on cellular immune responses. In...
Thus, HLA-E- Mtb -specific T cells have a highly diverse TCR repertoire.Linda VoogdAnne M.H.F. DrittijCalinda K.E. DingenoutsKees L.M.C. FrankenVincent van UnenKrista E. van MeijgaardenPaula RuibalRenate S. HagedoornJudith A. Leitner...
Reagent for direct detection of antigen-specific T cells. MHC tetramer technology is based on the ability of MHC-peptide complexes to recognize the antigen-specific T cells at a single cell level.
Tetramer-visualized gluten- specific CD4+ T cells in blood as a potential diagnostic marker for coeliac disease without oral gluten challenge. United Eur Gastroenterol J 2014;2:268-78.Christophersen, A., M. Ra´ki, E. Bergseng, K. E. Lundin, J. Jahnsen, L. M. Sollid, and S. W....
Nur, H. et al. (2013). “Preclinical Evaluation of Invariant Natural Killer T Cells in the 5T33 Multiple Myeloma Model” PLoS ONE 2013 8(5): e65075. Figure 4.来自幼稚和 5T33MM 小鼠的血液、BM、脾脏和肝脏的鼠 iNKT 细胞的代表性 FACS 分析。 活细胞(7-AAD 阴性)用 a-GalCer/CD1d 四聚体...