Ream, C. Lawrence, and T. Braciale. 2005. Transient loss of MHC class I tetramer binding after CD8+ T cell activation reflects altered T cell effector function. J. Immunol. 175.Drake DR 3rd, Ream RM, Lawrence CW, Braciale TJ (2005) Transient loss of MHC class I tetramer binding ...
Our HLA Class I tetramer has a mutation in the α3 domain on HLA. It minimizes undesirable binding to CD8 molecules and dramatically improves specificity to detect target T cells. CD8 is known to bind HLA to assist in TCR and HLA binding. This means that HLA contains a CD8-binding site....
Altman等证实了利用MHC Class I/多肽复合物四聚体作为探针,可以定量检测抗原特异性CTL(Science 1996, 274: 94-96)。在此之前,抗原特异性T细胞原本只能通过细胞因子的产生和细胞毒性活性的确认等间接方式检测。而MHC四聚体这项突破性的技术,不仅使抗原特异性T细胞可以被直接检测和分离,也可以实现对其功能与表型等的...
The figure shows live cells and demonstrates the sensitivity and increase in mean fluorescence intensity when cells are stained with Pentamer vs. tetramer. Further detail on Pro5® MHC Class I Pentamers and related products Pro5® Pentamer Product Size Options Pro5® Pentamers are available...
MHC-抗原肽复合物(即pMHC)向T细胞呈递抗原肽(表位),可特异性结合T细胞表面受体(TCR)。TCR在T细胞表面通常以多个相同拷贝(>1×105)表达。其中,CD8+T细胞主要识别由I类MHC分子呈递的抗原肽,而CD4+T细胞则主要识别由II类MHC分子呈递的抗原肽。其中:人类MHC→H...
BioLegend #280051 Flex-T™ Biotin H-2 K(b) OVA Monomer (SIINFEKL)是小鼠MHC复合体H-2预载OVA肽段(SIINFEKL)的Monomer,它可以结合并筛选OVA肽段(SIINFEKL)特异性的小鼠CD8+T毒性细胞。SIINFEKL与含有荧光标记的Tetramer预载...
Identification of CD8+ T cell epitopes is critical for the development of immunotherapeutics. Existing methods for major histocompatibility complex class I (MHC class I) ligand discovery are time intensive, specialized and unable to interrogate specific proteins on a large scale. Here, we present Epi...
1f). These data demonstrate that TCR-MAP is a specific and sensitive system to capture cognate pMHC–TCR interactions for both class I-restricted (HLA-A2) and class II-restricted T cells in humans. TCR-MAP captures mouse MHC class I-restricted (H2-Kb) and class II-restricted (H2-IAb) ...
MHC tetramer binding was evaluated using MHC skeleton (W6/32) targeting antibodies. Proper binding curves were observed using both antibodies, ensuring good specificity and affinity. Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) with Anti-HLA Class I Antibody (W6/32), Human IgG1. Cat. No...
•T细胞识别抗原的物质基础是其表面的TCR•TCR识别的配体是抗原肽-MHC分子复合物(pMHC)•TCR识别需要CD4/CD8分子作为共受体 AntigenrecognitionbyTcellsrequirespeptideantigensandpresentingcellsthatexpressMHCmolecules MHC分子提呈抗原肽供T细胞识别 一、T细胞抗原受体（TCR）•结构•...