MHC class-I tetramer stainings of HIV- and CMV-specific CD8+ T cells in chronic HIV infection.Marcus BuggertJohanna TauriainenTakuya YamamotoJuliet FrederiksenMartin A. IvarssonJakob MichaëlssonOle LundBo HejdemanMarianne JanssonAnders Sönnerborg...
MHC I 类五聚体专为这些目标而设计。它们直接结合特定特异性的 T 细胞受体,由主要组织相容性复合体 (MHC) 等位基因和肽组合决定。Pro5® 五聚体可用于检测和分离抗原特异性 CD8+ T 细胞群,少至 0.02% 的淋巴细胞。它们也适用于详细的表位表征和进一步的免疫监测。
Assessment of CD4(+) and CD8 (+) T cell responses using MHC class I and II tetramers. Methods Mol. Biol. 2013, 979, 71-79.Kurtulus S, Hildeman D. Assessment of CD4(+) and CD8 (+) T cell responses using MHC class I and II tetramers. Methods Mol Biol. 2013; 979 :71–79. ...
③ 在每个试管中加入50µL PBMC样品(例如,2x10⁶ cells/tube),室温孵育5分钟。 ④ 加入10µL T-Select MHC四聚体,轻柔地涡旋混匀,在2-8°C或室温(15-25°C)下避光孵育30-60分钟。 ⑤ 加入细胞分选抗体(例如anti-CD8抗体)并轻柔地涡旋混匀,在2-8°C中避光孵育30分钟。 ⑥加入3mL PBS或FCM缓冲...
Pro5® MHC Class I Pentamersthe most consistent, most published commercial technology for detecting antigen-specific CD8+ T cellsPro5® MHC Pentamer. The beautiful rational design of Pro5® MHC Pentamers presents five MHC peptide complexes in a plane at one end of their pentameric coiled...
MHC-抗原肽复合物(即pMHC)向T细胞呈递抗原肽(表位),可特异性结合T细胞表面受体(TCR)。TCR在T细胞表面通常以多个相同拷贝(>1×105)表达。其中,CD8+T细胞主要识别由I类MHC分子呈递的抗原肽,而CD4+T细胞则主要识别由II类MHC分子呈递的抗原肽。其中:人类MHC→H...
BioLegend #280051 Flex-T™ Biotin H-2 K(b) OVA Monomer (SIINFEKL)是小鼠MHC复合体H-2预载OVA肽段(SIINFEKL)的Monomer,它可以结合并筛选OVA肽段(SIINFEKL)特异性的小鼠CD8+T毒性细胞。SIINFEKL与含有荧光标记的Tetramer预载...
Identification of CD8+ T cell epitopes is critical for the development of immunotherapeutics. Existing methods for major histocompatibility complex class I (MHC class I) ligand discovery are time intensive, specialized and unable to interrogate specific proteins on a large scale. Here, we present Epi...
1f). These data demonstrate that TCR-MAP is a specific and sensitive system to capture cognate pMHC–TCR interactions for both class I-restricted (HLA-A2) and class II-restricted T cells in humans. TCR-MAP captures mouse MHC class I-restricted (H2-Kb) and class II-restricted (H2-IAb) ...
Toxin-coupled MHC class I tetramers can specifically ablate autoreactive CD8+ T cells and delay diabetes in nonobese diabetic mice. There is compelling evidence that self-reactive CD8(+) T cells are a major factor in development and progression of type 1 diabetes in animals and humans. ......