Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after tra... Quantifying Hydrogen Bond Cooperativity in Water: VRT Spectroscopy of the Water Tetramer Regulation of tryptase from human lung mast cells by heparin. Stabilization ...
When more than 75% of the exiting peptide is exchanged with a target peptide, the resulting tetramer can be used for CD8 + T cell staining. An exchange rate of higher than 90% corresponds to binding affinities in the low nanomolar range, while exchange rates lower than 65% are indicative ...
2.1.3. HCMV Peptide-Specific CD8 T Cell Responses Three pHLA tetramer complexes were used in conjunction with anti-CD8 antibodies for the immunostaining of conventional and unconventional HCMV peptide-specific CD8 T cell responses. Conventional CD8 T cells were detected using HLA-A*0201(A2)/pp65...