TEM-1 beta-lactamase is the most widespread enzyme to confer beta-lactam antibiotic resistance to gram-negative bacteria. It is a monomeric protein that requires no metals or cofactors for activity. TEM-1 beta-lactamase efficiently hydrolyzes most penicillin and cephalosporin substrates, including nit...
TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids ...
P7082-100mg Recombinant β-lactamase TEM-1 100mg - 说明书 1份 保存条件: -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。 注意事项: 由于...
Mutants of TEM-1 b-lactamase con- ferring resistance to ceftazidime. J Antimicrob Chemother 1989; 24: 103-10.Payne, D.J., Marriott, M.S. and Amyes, S.G. (1989) Mutants of the TEM-1 beta-lactamase conferring resistance to ceftazi- dime. J. Antimicrob. Chemother. 24, 103^110....
Beta Lactamase TEM 1 is an enzyme encoded by the bla gene in E. coli, with its expression being constitutive. The resistance to β-lactam antibiotics is directly proportional to the number of copies of the bla gene and the enzyme synthesized. ...
The structure of the 28 kDa beta-lactamase inhibitor protein-II (BLIP-II) in complex with the TEM-1 beta-lactamase has been determined to 2.3 A resolution... D Lim,HU Park,LD Castro,... - 《Nature Structural Biology》 被引量: 103发表: 2001年 A complex mutant of TEM-1 beta-lactamase...
CRYSTAL-STRUCTURE OF ESCHERICHIA-COLI TEM1 BETA-LACTAMASE AT 1.8-ANGSTROM RESOLUTION The X-ray structure of Escherichia coli TEM1 beta-lactamase has been refined to a crystallographic R-factor of 16.4% for 22,510 reflections between 5.0 and... C Jelsch,L Mourey,JM Masson,... 被引量: 202...
TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids ...
We engineered these mutations into the TEM-1 尾-lactamase to study their potential role in defining the substrate profile of the CARB enzymes. The mutations K234R and E240G in TEM-1 noticeably sixfold and twofold, respectively. The variant E240G also demonstrated an improved rate of second-...
The TEM-1 beta-lactamase is a globular protein containing 12 proline residues. The folding mechanism of this enzyme was investigated by kinetic and equilibrium experiments with the help of fluorescence spectroscopy and circular dichroism. The equilibrium denaturation of the protein induced by guanidine ...