TEM-1 beta-lactamase is the most widespread enzyme to confer beta-lactam antibiotic resistance to gram-negative bacteria. It is a monomeric protein that requires no metals or cofactors for activity. TEM-1 beta-lactamase efficiently hydrolyzes most penicillin and cephalosporin substrates, including nit...
P7082-100mg Recombinant β-lactamase TEM-1 100mg - 说明书 1份 保存条件: -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。 注意事项: 由于...
TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids ...
TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids ...
K. (2002). Non- covalent interaction energies in covalent complexes: TEM-1 beta-lactamase and beta-lactams. Proteins: Struct. Funct. Genet. 46, 86 - 96.Wang, X. J.; Minasov, G.; Shoichet, B. K. Noncovalent interaction energies in covalent complexes, TEM-1 beta-lactamase and beta- ...
Beta Lactamase TEM 1 is an enzyme encoded by the bla gene in E. coli, with its expression being constitutive. The resistance to β-lactam antibiotics is directly proportional to the number of copies of the bla gene and the enzyme synthesized. ...
We engineered these mutations into the TEM-1 尾-lactamase to study their potential role in defining the substrate profile of the CARB enzymes. The mutations K234R and E240G in TEM-1 noticeably sixfold and twofold, respectively. The variant E240G also demonstrated an improved rate of second-...
A TEM-1 beta-lactamase derivative containing the single amino acid substitution A237T slightly increased (from 24 to 32 microg/ml) the cephalothin MIC for ... J Blázquez,Negri, María-Cristina,Morosini, María-Isabel,... - 《Antimicrobial Agents & Chemotherapy》 被引量: 242发表: 1998年...
别名 Beta-lactamase TEM、IRT-4、Penicillinase + [9] 简介 TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hyd...
Structural and biochemical evidence that a TEM-1 beta-lactamase N170G active site mutant acts via substrate-assisted catalysis. J Biol Chem. 2009; 284 :33703–33712.BROWN, N. G., et al. Structural and biochemical evidence that a TEM- 1 beta-lactamase N170G active site mutant acts via ...