TEM-1 beta-lactamase is the most widespread enzyme to confer beta-lactam antibiotic resistance to gram-negative bacteria. It is a monomeric protein that requires no metals or cofactors for activity. TEM-1 beta-lactamase efficiently hydrolyzes most penicillin and cephalosporin substrates, including nit...
The crystal structure of the TEM1 β-lactamase has been determined at 1.9Å resolution by the molecular-replacement method, using the atomic coordinates of two homologous β-lactamase refined structures which show about 36% strict identity...
TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids ...
TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids ...
P7082-10mg Recombinant β-lactamase TEM-1 10mg P7082-100mg Recombinant β-lactamase TEM-1 100mg - 说明书 1份 保存条件: -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20...
We engineered these mutations into the TEM-1 尾-lactamase to study their potential role in defining the substrate profile of the CARB enzymes. The mutations K234R and E240G in TEM-1 noticeably sixfold and twofold, respectively. The variant E240G also demonstrated an improved rate of second-...
Structural and biochemical evidence that a TEM-1 beta-lactamase N170G active site mutant acts via substrate-assisted catalysis. J Biol Chem. 2009; 284 :33703–33712.BROWN, N. G., et al. Structural and biochemical evidence that a TEM- 1 beta-lactamase N170G active site mutant acts via ...
TEM-1 β-lactamase is one of the most common plasmid-mediated β-lactamases in Gram-negative bacteria. It has a broad spectrum of hydrolytic activity that includes penicillins and cephalosporins, but not extended-spectrum antibiotics (2). Extended-spectrum cephalosporins were developed, in part, ...
别名 Beta-lactamase TEM、IRT-4、Penicillinase + [9] 简介 TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hyd...
Lysine 234 is a residue highly conserved in all beta-lactamases, except in the carbenicillin-hydrolyzing enzymes, in which it is replaced by an arginine. Informational suppression has been used to create amino acid substitutions at this position in the broad spectrum Escherichia coli beta-lactamase ...