通过各种载体在体外将CAR基因转入T细胞中所制备的表达CAR分子的T细胞就被称为嵌合抗原受体T细胞(CAR-T cell)。CAR-T细胞可以根据其表面携带的特异性受体来追踪和识别并引导T细胞杀伤癌细胞。 单细胞测序(Single-cell sequencing)是指获取单个细胞遗传信息的测序技术,即在单个细胞水平上,对基因组、转录组、表观组等遗传...
上海交通大学景莹副及Texas A&M大学韩冷共同通讯在Cancer Cell在线发表题为“Expression of chimeric antigen receptor therapy targets detected by single-cell sequencing of normal cells may contribute to off-tumor toxicity”的研究论文,该研究利用了两个独立的包含多种正常人类组织的单细胞RNA测序(scRNA-seq)数据...
Single-cell RNA sequencingT cellsCD4CD8Smart-Seq2AlignmentClusteringGene expressionMarkersUnderstanding how populations of human T cells leverage cellular heterogeneity, plasticity, and diversity to achieve a wide range of functional flexibility, particularly during dynamic processes such as development, ...
6、对肿瘤区域的克隆型T细胞进行了分析,发现危重病人的克隆型Treg占据的比例更高,同时发现了这样一个亚群CD8+FOXP3+,分析克隆发现是,CD8+FOXP3-的细胞分化而来, Tregs and exhausted CD4 T cell clusters 共享很少,indicated the potential conversion from exhausted CD4 T cells to inducible Tregs。 最后看一下文...
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing Distinct microbial and immune niches of the human colon Single-cell RNA-seq enables comprehensive tumour and immune cell profiling in primary breast cancer A single-cell atlas of diffuse large B cell lymphoma ...
通过拟时间状态转换分析发现,T 细胞由初期、激活状态逐渐向耗竭状态进行转换(图 参考文献: Zheng C, Zheng L, Yoo J K, et al. Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single- Cell Sequencing.[J]. Cell, 2017, 169(7):1342-1356....
Single cell transcriptome analysis has become a compelling approach to decipher the properties of TILs, due to its ability to quantify gene expression and assemble TCR sequences simultaneously. In our recentNaturepaper, we have performed single cell RNA sequencing of 11,138 T cells isolated from pe...
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing 这篇文章[1]的一个亮点在于对肿瘤中的T细胞有了认识。 data 不得不说,这个样本的数量和质量真的太好了,一共是六个病人的外周血,正常组织,癌症区域配对样本,形成了非常好的对照。不知道是不是因为P1202病人T细胞的数量太少...
文章题目:Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing 研究团队:北京大学生命科学学院BIOPIC中心、北京未来基因诊断高精尖创新中心、北大-清华生命科学联合中心张泽民研究组,等。 发表时间:2017.7.15 期刊名称:Cell
Fig. 2 Targeted single-cell sequencing of four primary T-ALL samples 为了过滤低质量细胞,我们研究了每个细胞杂合性SNP的位点和等位基因脱落(ADO)率。将位点丢失率(LDO)定义为SNPS与<4 reads的比例。高LDO比例表明细胞中的许多SNP具有低覆盖率,表明基因组的较大区域未扩增。ADO表明,如果只有一个等位基因被扩增...