T cells express T cell receptors (TCRs) composed of somatically recombined TCRα and TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen complexes and drive the antigen-specific adaptive immune response to pathogens and cancer. The TCR repertoire in each ...
We next sought to determine if the persistence of expanded infected clonotypes was restricted to specific memory CD4+ T cell subsets. Since the memory phenotype of individual p24+ cells was recorded during cell sorting, we were able to analyze the distribution of HIV-infected expanded clonotypes ...
As these cells are located at the site of inflammation in CeD, they should harbor the highest density of disease-relevant γδ T cells. Our single-cell sequencing method enabled us to sequence the vast majority of all cells sorted from each individual. We found that there are clear ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
We performed high-throughput and paired γδ TCR sequencing of single intraepithelial γδ T cells of untreated CeD patients (=8; 1821 cells), CeD patients treated with a gluten-free diet (=5; 436 cells) and controls (=7; 1068 cells). We found that CeD patients, both untreated and ...
Abstract The unbiased, paired analysis of T-cell receptor (TCR) α- and β-chain usage at the single-cell level provides a valuable window of understanding into the TCR repertoire and the nature of the immune response. Earlier technologies for TCR repertoire …more ...
Muscle-invasive bladder cancer (MIBC) is a highly aggressive disease with a poor prognosis. B cells are crucial factors in tumor suppression, and tertiary lymphoid structures (TLSs) facilitate immune cell recruitment to the tumor microenvironment (TME).
为了在 TCR 谱系水平上“功能”验证上述转录组学观察结果,使用Scirpy来执行谱系映射以及对 CRC CD8+ T 细胞的 scTCR-seq 图谱进行抗原特异性预测。 Scirpy is a package to analyse T cell receptor (TCR) or B cell receptor (BCR) repertoires from single-cell RNA sequencing (scRNA-seq) data in Python...
Single-cell sequencing technologies can be used to detect the genome, transcriptome and other multi-omics of single cells. They can show the differences and evolutionary relationships of various cells. This review introduces the latest advances in single-cell sequencing technologies and their applications...
Human FOXP3+ regulatory T (Treg) cells are central to immune tolerance. However, their heterogeneity and differentiation remain incompletely understood. Here we use single-cell RNA and T cell receptor sequencing to resolve Treg cells from healthy individ