The present disclosure relates to compositions and methods for assessing extended length T-cell receptor (TCR) transcript sequences (i.e., TCR transcript sequences that span TCR transcript variable regions) in a spatially-defined manner across a tissue sample, specifically providing for obtaining ...
et al. The T cell receptor repertoire of tumor infiltrating T cells is predictive and prognostic for cancer survival. Nat. Commun. 12, 4098 (2021). Google Scholar Pai, J. A. & Satpathy, A. T. High-throughput and single-cell T cell receptor sequencing technologies. Nat. Methods 18, ...
T cell receptors (TCR). A typical T cell expresses one unique TCR, and the TCR is made up of two chains—the TCRα and TCRβ chains—that both determine the set of molecules that the T cell can respond to. Since T ...
Single-cell T-cell receptor sequencing of paired human atherosclerotic plaques and blood reveals autoimmune-like features of expanded effector T-cells Author links open overlay panelM.A.C. Depuydt 1, F. Schaftenaar 2, K.H.M. Prange 3, A. Boltjes 4, E. Hemme 5, L. Delfos 5, J. ...
Two single-cell sequencing libraries, either aiming to target up to 10,000 cells per chip or 30,000 cells per chip, were made. After perturbation index assignment and filtering multiplet cell data, 1,199 and 3,271 usable single cells were collected from the two batches, respectively. Therefor...
19 Such strategies provide deep samples of immune receptor repertoires (up to the order of 107 reads) but are unable to capture complete B cell receptor (BCR) or TCR variable regions due to the loss of pairing information. Biases quickly emerged toward sequencing only the heavy antibody chain/...
Compared with paired primary lesions, the hypomethylated probes in metastatic lesions were enriched in genes involved in chemokine signaling, T cell receptor signaling, inflammatory response, Th1 and Th2 cell differentiation, cytokine-cytokine receptor interaction, IL2-STAT5 signaling, B cell receptor ...
Glypican-3 (GPC3) is a heparan-sulfate proteoglycan frequently expressed on the cell membrane of malignant hepatocytes in hepatocellular carcinoma. The capacity for screening potential antibodies in vitro using human hepatocellular lines is critical to e
The present disclosure addresses these problems in the context of simultaneously amplifying and sequencing the transcripts of T cell receptor (TCR) alpha and beta chain peptides. However, the disclosed methods can be readily extended to other sets of target transcripts. In one aspect, the disclosure...
For examples, the T-cell receptor-β (TRB) V6-5, HLA-A*02:01 combination was associated with a positive outcome, and the TRBV6-1, HLA-A*01:01 combination was associated with a negative outcome. Overall, these results point to V or J usage, HLA allele combinations as survival ...