Abstract 1044: Single Cell RNA-sequencing Of Endothelial Cells In Atherosclerosis Reveals A Single Phenotypic Transition To A Mesenchymal Cell Statedoi:10.1161/atvb.44.suppl_1.1044AtherosclerosisEndothelialGenomicsIntroduction:Coronary artery disease (CAD) is the leading cause of mortality in the United ...
B. et al. Paired-cell sequencing enables spatial gene expression mapping of liver endothelial cells. Nat. Biotechnol. 36, 962–970 (2018). Article CAS PubMed PubMed Central Google Scholar MacParland, S. A. et al. Single cell RNA sequencing of human liver reveals distinct intrahepatic ...
et al. Mechanisms of diabetes-induced endothelial cell senescence: role of arginase 1. Int. J. Mol. Sci. 19, 1215 (2018). Article Google Scholar Kiss, T. et al. Single-cell RNA sequencing identifies senescent cerebromicrovascular endothelial cells in the aged mouse brain. Geroscience 42, ...
Cell Communication and Signaling(2024) PFKFB3 in neovascular eye disease: unraveling mechanisms and exploring therapeutic strategies Peiyu Liu Dandan Sun Fang Wei Cell & Bioscience(2024) Angiogenesis-on-a-chip coupled with single-cell RNA sequencing reveals spatially differential activations of autophagy ...
Traditional methods, such as fluorescence-activated cell sorting, allow analysis of tumor and TME cells via their physical separation; however, they show limitations in capturing the complexity of tumor ecosystems and cell interactions. Single-cell RNA sequencing (scRNA-seq) profiling has revealed ...
To investigate cell cycle state of endothelial cells within the continuum of arterial–venous phenotypes, we isolated endothelial cells from retinal vasculature of wild-type P6 and P15 mice and performed single-cell (sc)RNA sequencing using 10x Genomics and a drop-seq approach28. After filtering, ...
cell types may have limitations in revealing the full range of responses to such stimuli in the glomerular environment. Recent work using single-cell sequencing in DN models18,19and patients21have allowed detailed characterisation of the transcriptomic changes induced in distinct renal cell types ...
(refs.3,8,24,25,26,27). When analyzing isolated mouse brain cells by single-cell RNA sequencing (scRNA-seq), we identified 20 cell clusters, including two endothelial clusters (Extended Data Fig.3a,b). Some cells in the endothelial cell cluster 2 expressedAce2, albeit at a lower level ...
The role of glomerular endothelial cells (GEC) in the pathogenesis of renal diseases is not yet well defined and better understanding of their biology could lead to discoveries of new therapeutic targets1. Recently, transcriptomic-profiling studies and single cell RNA-sequencing of isolated glomeruli ...
Here we used paired-cell sequencing, in which mRNA from pairs of attached mouse cells were sequenced and gene expression from one cell type was used to infer the pairs' tissue coordinates. We applied this method to pairs of hepatocytes and liver endothelial cells (LECs). Using the spatial ...