SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是最早被认识到的mRNA多腺苷化信号[1], 90%的真核mRNA 的AATAAA 序列都在距剪切位点的30 个核苷酸的范围内[2], 它指导...
SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱如下: EcoRI XhoI SacI MluI HindIII EcoRV 411 TTTTGCAAAA AGCTCCTCGA GGAGCTCACG
多腺苷化信号又称PolyA信号(Polyadenylationsignal), AAUAAA(在基因组序列中表示为AAl’AAA)是其主要形式, 参与真核mRNA 3’端形成的重要过程。己知Alu序列和PolyA 信号与多种生物学现象关联,如Alu的插入或缺失可引起肿 瘤、染色体重组等;而一大类导致地中海贫血的血红蛋白病 ...
DNA序列(240bp),含有AATAAA六核苷酸多腺苷化信号(Polyadenylationsignal)。在pEGFP-C1质粒的GFP 基因下游插入14个同向串联的Alu序列(Alu14),构建pAlu14质粒,瞬时转染HeLa细胞,用Northernblot检测 和荧光显微镜观察GFPRNA和GFP蛋白表达,发现Alu串联序列强烈抑制GFP基因表达,该序列没有转录终 ...
SV40 PolyA(猴空泡病毒PolyA,简称PolyA)序列是有转录终止作用和使转录的mRNA添加PolyA尾的DNA序列(240 bp),含有AATAAA六核苷酸多腺苷化信号(Polyadenylation signal)。在pEGFP-C1质粒的GFP基因下游插入14个同向串联的Alu序列(Alu14),构建pAlu14质粒,瞬时转染HeLa细胞,用Northern blot检测和荧光显微镜观察GFP RNA和GFP蛋白...
The soluble neuropilin-1 (sNRP-1) gene employs an extremely short dual function polyadenylation (pA) signal/stop codon that is efficient for termination of gene transcription and translation in vivo. However, the functionality and usefulness of this signal in regard to other genes is unknown. Thi...
SV40 late polyadenylation signal1731-1932 β-lactamase (Ampr) coding region2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱见说明书:https://www.beyotime.com/Manual/D2762 pRL-SV40-N(报告基因质粒).pdf。 pRL-SV40-N中没有的酶切位点(Restriction enzymes that do not...
SV40 late polyadenylation signal1731-1932 β-lactamase (Ampr) coding region2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱见说明书:https://www.beyotime.com/Manual/D2762 pRL-SV40-N(报告基因质粒).pdf。 pRL-SV40-N中没有的酶切位点(Restriction enzymes that do not...
SV40 late polyadenylation signal1781-1942 β-lactamase (Ampr) coding region2079-2494 pRL-SV40-C质粒(3775bp)的图谱如下: pRL-SV40-C的多*位点的详细图谱如下: EcoRI XhoISacIMluIHindIIIEcoRVSalI 1631TAATTCTAGGCTCGAGGAGCTCACGCGTAAGCTTGAATTCGATATCGTCG ...
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substr...