河南大学医学院生物化学与分子生物学教研室,开封 475004 摘要: SV40 PolyA(猴空泡病毒 PolyA, 简称 PolyA)序列是有转录终止作用和使转录的 mRNA 添加 PolyA 尾的DNA 序列(240 bp), 含有 AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。在 pEGFP-C1 质粒的 GFP基因下游插入 14 个同向串联的 Alu 序列(...
The soluble neuropilin-1 (sNRP-1) gene employs an extremely short dual function polyadenylation (pA) signal/stop codon that is efficient for termination of gene transcription and translation in vivo. However, the functionality and usefulness of this signal in regard to other genes is unknown. Thi...
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substr...