[ Abstract] Objective ToinvestigatetheregulatoryeffectsoftwokindsofSV40poly(A)signalsontheupstreamgeneexpressioninthreecelllines, soastoprovidetheoreticalevidenceforselectionofpoly(A)ofvectors.Methods AdualluciferasereportervectorDual-Lucwasconstructed, andtwoSV40poly(A)signalsequenceswereinsertedinthedownstreamof...
SV40PolyA(SimianVires40Polyadenylation.Signal,PolyA)是猴巨细胞病毒早期蛋白多聚腺苷酸加尾序列(240bp),含有SV40早期蛋白加尾信号,是一种强的真核细胞转录终止序列。一般认为PolyA位置下游序列与其上游序列不再属于同一基因,而属于基因侧翼序列。有人证明L1.2ORF2插入GFP基因下游,反序表达高于正序。本文拟用L1重复序列...
Surgery Hospita, l Second M ilitary M edicalUniversity, Shanghai200438, China [Abstract] Objective To investigate the regulatory effects of two kinds ofSV40 poly(A) signalson the upstream gene expressionin three cell lines, so as to provide theoretical evidence for selection of poly(A) of ...