SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱如下: EcoRI XhoI SacI MluI HindIII EcoRV 411 TTTTGCAAAA AGCTCCTCGA GGAGCTCACG
常见的PAS tk polyA, SV40 late and early polyA or BGH polyA mammalian terminators (SV40, hGH, BGH, and rbGlob) SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是...
SV40 late polyadenylation signal1731-1932 β-lactamase (Ampr) coding region2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱见说明书:https://www.beyotime.com/Manual/D2762 pRL-SV40-N(报告基因质粒).pdf。 pRL-SV40-N中没有的酶切位点(Restriction enzymes that do not...
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substr...
SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱见说明书:https://www.beyotime.com/Manual/D2762 pRL-SV40-N(报告基因质粒).pdf。 pRL-SV40-N中没有的酶切位点(Restriction enzymes tha...
SV40 late polyadenylation signal1130-1291 β-lactamase (Ampr) coding region1438-2298 pGLuc-Dura-SV40-C质粒(3124bp)的图谱如下: pGLuc-Dura-SV40-C的多克隆位点的详细图谱见说明书:https://www.beyotime.com/Manual/D2770 pGLuc-Dura-SV40-C (报告基因质粒).pdf ...
SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多*位点的详细图谱如下: EcoRI XhoI SacI MluI HindIII EcoRV 411 TTTTGCAAAA AGCTCCTCGA GGAGCTCACG CGTAAGCTTG AATTCGATAT AAAACGTTTT TCGAGGAGCT CCTCGAGTGC GCAT...
SV40 late polyadenylation signal 1781-1942 β-lactamase (Ampr) coding region 2079-2494 pRL-SV40-C质粒(3775bp)的图谱如下: pRL-SV40-C的多克隆位点的详细图谱见说明书:https://www.beyotime.com/Manual/D2768 pRL-SV40-C (报告基因质粒).pdf。 pRL-SV40-C中没有的酶切位点(Restriction enzymes ...
目的:在人类基因组中,有98%的DNA序列属于非编码序列,非编码序列中大约有一半的序列属于重复序列,Alu是人类基因组中除LINE-1(long interspersed nuclear elements1,长散在重复序列核元件1)以外最主要的重复序列家族.多腺苷化信号又称PolyA信号(Polyadenylation signal),AAUAAA(在基因组序列中表示为AATAAA)是其主要形式,...
"Terminator" is intended to include whatever is required in such sequences, not necessarily limited to the polyadenylation. function. This definition is necessitated by the current state of knowledge in the art, wherein the relevance of sequences in addition to the polyadenylation signal is unclear...