常见的PAS tk polyA, SV40 late and early polyA or BGH polyA mammalian terminators (SV40, hGH, BGH, and rbGlob) SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是...
site or the late SV40 signal, suggesting that alterations in the strength of the upstream poly(A) signal influence expression of viral replication factors... SS Terhune,WG Hubert,JT Thomas,... - 《Journal of Virology》 被引量: 117发表: 2001年 Vargula hilgendorfii luciferase: a secreted repo...
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substr...
The poly A tail is involved in mRNA stability and is a prerequisite to export from the nucleus to the cytoplasm, where translation occurs. A G/U-rich region is usually situated downstream of the consensus signal sequence. There is evidence to suggest that this G/U region is involved with ...
SV40 late polyadenylation signal 1671–1872 β-lactamase (Amp r ) coding region 2019–2879 Note: –^–, position of intron; Rluc, cDNA encoding the Renilla luciferase enzyme; Amp r , gene conferring ampicillin resistance in E. coli; ori, origin of plasmid replication in E. coli. Arrows wit...
The mouse DNA fragment, which contains an intervening sequence and a poly(A) addition site, has been inserted in both possible orientations relative to the SV40 late region promoter. While the fragment is transcribed regardless of orientation, the RNA splice signal and poly(A) addition site are...
The regulatory region includes the origin of replication (ori), the early and late promoters, the enhancer, and the packaging signal, ses. The enhancer is composed of two 72 bp direct repeats. 3. The viral life cycle Soon after infection, the virus produces T-antigens from the early ...
Mutations in GC-motifs I and II do not decrease the activity of the late-early promoter, whereas mutations in the GC-motifs III-VI have a moderate effect on it. The in vivo phenotype of the GC-motif mutants can be almost fully reproduced in vitro using a nuclear extract. DNase I ...