M ethods A dual luciferase reporter vectorDua- lLuc was constructed, and two SV40 poly(A) signal sequenceswere inserted in the downstream of the R-Luc gene separately. Thenthe t wo diverse dual luciferase reporter gene vectors Dua- lLuc2 and Dua- lLuc3 were transfected into 293, L-02...
多腺苷化信号又称PolyA信号(Polyadenylationsignal), AAUAAA(在基因组序列中表示为AAl’AAA)是其主要形式, 参与真核mRNA 3’端形成的重要过程。己知Alu序列和PolyA 信号与多种生物学现象关联,如Alu的插入或缺失可引起肿 瘤、染色体重组等;而一大类导致地中海贫血的血红蛋白病 都归因于AAUA八A这个序列的点突变。本...
SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是最早被认识到的mRNA多腺苷化信号[1], 90%的真核mRNA 的AATAAA 序列都在距剪切位点的30 个核苷酸的范围内[2], 它指导...
(A) of vectors.Methods A dual luciferase reporter vector Dual-Luc was constructed,and two SV40 poly(A) signal sequences were inserted in the downstream of the R-Luc gene separately.Then the two diverse dual luciferase reporter gene vectors Dual-Luc2 and Dual-Luc3 were transfected into 293,...
A thalassemia caused by a polyadenylation signal mutation. Nature This idea is strengthened by observations on the effect of deletion mutations in or around the AATAAA sequence on polyadenylation of late simian virus 40 (SV40) mRNA; removal of this sequence prevents poly(A) addition. Naturally .....
关键词:非编码序列;GFP;茎环结构;Alu ;SV40PolyA Detection and sequence analysis of an imperfect stem-loop structure in cis activating gene element from SV40PolyA 1,2 1 3 3 1 WANG Hong-Gang , MA Huan , LI Zhu , ZHANG Bin , JING Xiang-Yang , 1 1 ZHANG Yuan , LU Zhan-Jun 1. ...
pGL4.73[hRluc /SV40]荧光报告质粒别名: pGL4.73启动子: SV40复制子: pUC ori,SV40 ori终止子: SV40 poly(A) signal质粒分类: ...
关键词:Alu;SV40PolyA;AATAAA信号;GFP;转录终止 TheeffectsofSV40PolyAsequenceanditsAATAAAsignalonup- streamGFPgeneexpressionandtranscriptiontermination LIShu-Ping 1,2 ,FENGJing-Jing 3 ,WANGHong-Gang 4 ,WANGXiu-Fang 1 ,LVZhan-Jun 1 1.DepartmentofGenetics,HebeiKeyLabofLaboratoryAnimal,HebeiMedical...
关键词:Alu;SV40 PolyA:多腺苷化信号;GFP 英文摘要 SV40 and antisense Senseor PolyAsequence influence GFP AATAAA upstreamreportgene signal expression ABSTRACT human the Objective:In genome,98%DNAsequence tothe ahalf belongs non-codingproteinsequences.Nearly are the non—codingproteinsequences repetitive...
pRL-SV40哺乳报告质粒信息 启动子: SV40,T7 复制子: pUC ori,SV40 ori 终止子: SV40 poly(A) signal 质粒分类: 哺乳细胞,信号通路报告载体 质粒大小: 3705bp 原核抗性: Amp 克隆菌株: DH5α 培养条件: 37℃,有氧 LB