M ethods A dual luciferase reporter vectorDua- lLuc was constructed, and two SV40 poly(A) signal sequenceswere inserted in the downstream of the R-Luc gene separately. Thenthe t wo diverse dual luciferase reporter gene vectors Dua- lLuc2 and Dua- lLuc3 were transfected into 293, L-02...
多腺苷化信号又称PolyA信号(Polyadenylationsignal), AAUAAA(在基因组序列中表示为AAl’AAA)是其主要形式, 参与真核mRNA 3’端形成的重要过程。己知Alu序列和PolyA 信号与多种生物学现象关联,如Alu的插入或缺失可引起肿 瘤、染色体重组等;而一大类导致地中海贫血的血红蛋白病 ...
SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是最早被认识到的mRNA多腺苷化信号[1], 90%的真核mRNA 的AATAAA 序列都在距剪切位点的30 个核苷酸的范围内[2], 它指导...
sequence that was deleted AATAAA signal in sense or antisense orientation were inserted between GFP and Alu tandem sequence in pAlu14. The results showed that all the inserted PolyA sequences partly eliminated the inhibition induced by Alu14. PolyA sequences without AATAAA signal in sense or antise...
pRL-SV40哺乳报告质粒信息 启动子: SV40,T7 复制子: pUC ori,SV40 ori 终止子: SV40 poly(A) signal 质粒分类: 哺乳细胞,信号通路报告载体 质粒大小: 3705bp 原核抗性: Amp 克隆菌株: DH5α 培养条件: 37℃,有氧 LB
(A) of vectors.Methods A dual luciferase reporter vector Dual-Luc was constructed,and two SV40 poly(A) signal sequences were inserted in the downstream of the R-Luc gene separately.Then the two diverse dual luciferase reporter gene vectors Dual-Luc2 and Dual-Luc3 were transfected into 293,...
The poly A tail is involved in mRNA stability and is a prerequisite to export from the nucleus to the cytoplasm, where translation occurs. A G/U-rich region is usually situated downstream of the consensus signal sequence. There is evidence to suggest that this G/U region is involved with ...
A thalassemia caused by a polyadenylation signal mutation. Nature Most eukaryotic messenger RNAs have the sequence AAUAAA 11-30 nucleotides from the 3'-terminal poly(A) tract. Since this is the only significant sequence homology in the 3' non-coding region it has been suggested that it may be...
The mouse DNA fragment, which contains an intervening sequence and a poly(A) addition site, has been inserted in both possible orientations relative to the SV40 late region promoter. While the fragment is transcribed regardless of orientation, the RNA splice signal and poly(A) addition site are...
Confirmation of 17kT- and LT-regulated hepatic mRNAs identified by array analysis was obtained by semi-quantitative RT–PCR using primers sequences and PCR conditions described in Supplementary Table S5. Preparation of liver proteins, liver RNA, immunoblotting and northern blotting Preparation of ...