Poly(A) signals. 来自 ResearchGate 喜欢 0 阅读量: 182 作者: N Proudfoot 摘要: Author information: (1)Sir William Dunn School of Pathology, University of Oxford, England.关键词:Animals Mammals Saccharomyces cerevisiae Chromosome Deletion Poly A Signal Transduction Base Sequence Molecular Sequence Data...
(A) signal sequences were inserted in the downstream of the R-Luc gene separately.Then the two diverse dual luciferase reporter gene vectors Dual-Luc2 and Dual-Luc3 were transfected into 293,L-02,and HeLa cells.The relative quantities of the target gene (F-Luc) to control gene (R-Luc)...
We found that the gene segment containing the μ m poly(A) signals, along with 536 bp of downstream flanking sequence, acted as a transcription terminator in both myeloma cells and L cell fibroblasts. Neither a 141-bp DNA fragment (which directed efficient polyadenylation at the μ m site),...
(A)signalsontheupstreamgeneexpressioninthreecelllines, soastoprovidetheoreticalevidenceforselectionofpoly(A)ofvectors.Methods AdualluciferasereportervectorDual-Lucwasconstructed, andtwoSV40poly(A)signalsequenceswereinsertedinthedownstreamoftheR-Lucgeneseparately.ThenthetwodiversedualluciferasereportergenevectorsDual-...
In contrast, a more refined series of mutations, in which clusters of 3 bases were replaced, failed to cause loss of function. We conclude that the downstream element of the mouse beta globin poly(A) signal is bipartite in structure, and that portions of its sequence are functionally ...
A Simple Decision Rule for Recognition of Poly ( A ) Tail Signal Motifs in Human Genome KAUST Repository A Simple Decision Rule for Recognition of Poly ( A ) Tail Signal Motifs in Human Genome KAUST RepositoryBackground is the numerous attempts were made to predict motifs in genomic sequences...
Motivation: Polyadenylation is the addition of a poly(A) tail to an RNA molecule. Identifying DNA sequence motifs that signal the addition of poly(A) tails is essential to improved genome annotation and better understanding of the regulatory mechanisms and stability of mRNA. Existing poly(A) moti...
Class V (32% of the total) contained no known poly (A) signal. Analysis of the relative frequency of sequences between 1 and 60 nucleotides upstream from the poly (A) tail was determined for each cDNA sequence. Overall, the 60... AJ Wood,RJ Duff,Q Zeng,... - 《Bryologist》 被引...
Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full...
Poly(A) sites are defined Correspondence: Martine Simonelig Tel: +33 4 34 35 99 59; Fax: +33 4 34 35 99 57 E-mail: Martine.Simonelig@igh.cnrs.fr by two sequences, the canonical poly(A) signal AAUAAA localized upstream of the cleavage site and a downstream U/GU-rich motif; both...