因此,“序列同一性百分比(percentage of sequence identity)”如下计算: 通过在比较窗内比较两个最佳比对的序列, 确定两个序列中出现相同的核酸碱基(例如, A、T、C、G、I)或相同的氨基酸残基(例如,Ala、 Pro、 Ser、 Thr、 Gly、 Val、 Leu、 Ile、 Phe、 Tyr、 Trp、 Lys、 Arg、 His、 Asp、 Glu、 ...
Promoters containing multiple copies of HRE sequences have been shown to drive specific gene expression in hypoxia. This HRE-directed gene expression has been well established in multiple systems, including hypoxia-specific chimeric antigen receptor (CAR)-T cells and multiple other therapies.9,10 ...
CONSTITUTION: A vector HSV-TK-ires-PNP(KCTC 10205BP) co-expressing HSV-TK and PNP enzymes comprises (a) a promoter suitable for a target cell, (b) a DNA sequence encoding HSV-TK protein, (c) an ires site, (d) a DNA sequence encoding PNP protein, and (e) a poly-A signal, where...
The chemiluminiscent signal was captured using Ge- neGenome HR imaging system (Syngene). Chromatin immunoprecipitation The protocol was described previously [27]. The sequences of PCR primers: ICP0 (promoter) ChIP: 5′-TAA TGG GGT TCT TTG GGG GAC ACC-3′ and 5′-TGC AAA TGC GAC CAG ...
BX-795 is an inhibitor of 3-phosphoinositide-dependent kinase 1 (PDK1), but also a potent inhibitor of the IKK-related kinase, TANKbinding kinase 1 (TBK1) and IKKɛ. In this study we attempted to elucidate the molecular mechanism(s) underlying the inhi
Because these drugs specifically target HSV thymidine kinase (TK), drug-resistant mutants can arise in immunocompromised individuals[2, 3]. Eukaryotic cells respond to environmental stress and stimuli by recruiting or activating signal transduction pathways. The c-Jun NH2-terminal kinase (JNK) and ...
A recombinant herpes simplex virus (HSV) viral vector genome which has substantially lost its transducing properties as a result of a DNA sequence change in the gene coding for Vmw65 protein and also
This sequence contains a single open reading frame on the r -strand consistent with the known polarity of TK mRNA synthesis and between recognizable transcription initiation (TATTAA) and poly A (AATAAAA) signals. Hybridization and S1 nuclease mapping of poly A-containing RNA (synthesized at early...
In addition, sequence analysis of many of the commonly used HSV-1 strains reveal differences, some of the most dramatic of which are in the LAT region. Furthermore, as the functionality of at least some HSV-1 proteins varies among different models of infection [10] the conclusions drawn ...