CONSTITUTION: A vector HSV-TK-ires-PNP(KCTC 10205BP) co-expressing HSV-TK and PNP enzymes comprises (a) a promoter suitable for a target cell, (b) a DNA sequence encoding HSV-TK protein, (c) an ires site, (d) a DNA sequence encoding PNP protein, and (e) a poly-A signal, where...
NTC8485载体图谱显示第一合成编码序列(A)O2-gD2和(B)O2-Ubi-gD2tr的位置。[0040]图2显示在施用500μg剂量的COR-1疫苗后受试者的注射部位的照片。(A)立即;(B)注射后45分钟; (C)注射后24小时; 和(D)注射后48小时拍摄右臂注射部位的照片。[0041]图3显示在施用500μg剂量的COR-1疫苗后受试者的注射...
(D) Same as in (C), except the luciferase signal is normalized to the CMV backbone. Data are represented as mean ± SD and are representative of n = 3 biological replicates. See also Figure S1. Hypoxia-specific expression of HSV-tk We initially tested the HRE-driven system with the ...
opposite to that of TK was selected and designated pMJ27. Plasmid pMJ27 contains unique XbaI and HindIII sites flanking the SV40 promoter plus enhancer, and has an unique XhoI site downstream of the SV40 polyadenylation signal. The human cytomegalovirus (HCMV) Towne strain enhancer was cloned ...
References 1 Goldenberg D, Mador N, Ball MJ, Panet A, Steiner I. The abundant latency-associated transcripts of herpes simplex virus type 1 are bound to polyribosomes in cultured neuronal cells and during latent infection in mouse trigeminal ganglia. J Virol 1997; 71:2897-2904. 2 Javier ...
Because these drugs specifically target HSV thymidine kinase (TK), drug-resistant mutants can arise in immunocompromised individuals[2, 3]. Eukaryotic cells respond to environmental stress and stimuli by recruiting or activating signal transduction pathways. The c-Jun NH2-terminal kinase (JNK) and ...
Because these drugs specifically target HSV thymidine kinase (TK), drug-resistant mutants can arise in immunocompromised individuals2,3. Eukaryotic cells respond to environmental stress and stimuli by recruiting or activating signal transduction pathways. The c-Jun NH2-terminal kinase (JNK) and p38 ...
This sequence contains a single open reading frame on the r -strand consistent with the known polarity of TK mRNA synthesis and between recognizable transcription initiation (TATTAA) and poly A (AATAAAA) signals. Hybridization and S1 nuclease mapping of poly A-containing RNA (synthesized at early...
It is unclear whether the switching of histone methyltransferase with demethylase activity on bivalent marks is cell signal specific or a result of high levels of PRC1/PRC2 within a cell—the maintenance of which is subverted by the HSV-1 LAT. 6. Summary and Discussion The analysis of HSV-...