In particular, a signal sequence is also included such that the post-translational processing is effected in the secretory pathway of plants. Suitable linker sequences and DNA constructs for use in the method are also described.doi:WO2000011175 A1WILLEM FRANS BROEKAERT...
alternately.The function of RNA is closely related、蕊tll its structure and structures are more conservative.This paper reports a method for accurately identifying the secondary structure of poly(A)site sequences in plants.First,Vienna package Was used to convert the sequences to the dot—bracket ...
orenvironmentalcues.Thepositionofapoly(A)siteispredeterminedbysignalsinthepre.mRNAsequencethatarerecognizedbyacomplexofpoly(A)factors.Formorepoly(A)sitesconditions,aspecificpoly(A)factormutantwouldresultindifferentpoly(A)sitetobeselected,andcauseexpressiondifferencesintheformsoftranscripts,SOitisamajorimpactforthe...
Based on the peptide sequence of the purified protein, we have identified the p110 as the poly(ADP-ribose) polymerase (PARP). Using bandshift experiments and footprinting studies, we present evidence that PARP is a component of protein complexes bound to the EP enhancer that increases the on ...
a protein encoding sequence coding for more than one protein and a 3'-terminator region which includes a polyadenylation signal, each of the protein encoding sequences being separated from an adjacent protein encoding sequence by a DNA sequence which on translation provides a cleavage site whereby ...
in the N-terminal which was considered to be a signal peptide.The protein exhibits a homology of 99% with PGIP in 'Granny Smith','Fuji'and 'Golden Delicious'apple.The SDS-PAGE indicated that the size of expressed protein was consistent with that of expected protein.【Conclusion】A pgip ...
(A)位点的特征提取和识月Ⅱ算法wereimprovedfromO.81andO.84oftheoriginalmodelto0.88and0.91.respectivelyreachingthelevelofefficientlyassistingwetexperimentsinidentifyingpoly(A)sites.ToshowtheefficacyofPAS,wetestedafewmRNAsequencesincludingthosewithmultiplepoly(A)sites.Moreinterestingly,thepoly(A)siteefficienciesof...
However, we are of the opinion that suitably used signal processing methods can help in developing more efficient prediction models in bioinformatics and computational biology. In this study, we focus on the computational recognition of human PAS in genomic DNA. PAS are hexamer sequences located ...
Whether one of the ncPAPs is involved in heterochromatin assembly in plants remains to be determined. Another process yet to be explored in plants is the activation of translation by cytoplasmic polyadenylation or uridylation of latent mRNAs, a process that has been described in S. pombe, ...
A protein kinase first described in Chlamydomonas flagella has recently been shown to operate in primary cilia as well. CALK (a Chlamydomonas aurora-like kinase) translocates into the flagella in a kinesin-2-dependent manner (Pan and Snell, 2003) when cells receive a signal telling them to shor...