The present invention relates to heterologous genes comprising non-plant 3′ termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells...
The major polyadenylation signal (PAS) represents a nucleotide hexamer that adheres to the AATAAA consensus sequence. Over a half of human genes have multiple cleavage and polyadenylation sites, resulting in a great diversity of transcripts differing in function, stability, and translational activity. ...
Alpha-thalassaemia caused by a polyadenylation signal mutation. Most eukaryotic messenger RNAs have the sequence AAUAAA 11–30 nucleotides from the 3′-terminal poly(A) tract 1,2 . Since this is the only significant sequence homology in the 3′ non-coding region it has been suggested that it ...
the maturation of messenger RNA precursors (pre-mRNAs), involving the processing of 3’termini, is critical for mRNA function and stability [1]. In the processing of the 3’termini, the 3’end of nascent mRNA is cleaved, followed by addition of a poly(A) tail (i.e., polyadenylation). ...
stability or transport of the mRNA out of the nucleus. Signals near the 3′ extremity of the mature mRNA mark positions at which polyadenylation occurs. The consensus signal is AUAAA (seeFig. 1.4). Mutations in the poly-A signal sequence have been shown to cause thalassemia (seeChapter 40)...
This results in the acquisition of a poly (A) tail by activation of downstream poly (A) signal sequence that will then provide enhanced mRNA stability by increasing its half-life (Marzluff et al., 2008). The increased half-life of the polyadenylated H3.1 mRNA is due to the poly (A) ...
Changes in polyadenylation site recognition sequences in proximal versus distal polyadenylation sites for genes undergoing APA with quiescence.aSchematic showing the relative position of the UGUA motif, hexamers, the cleavage site and GU/U-rich motifs.bThe frequencies with which different possible hexame...
For kallisto, a transcriptomic reference was also generated using the GRCm39 reference sequence and GENCODE M28 annotations. The default k-mer length of 31 was used to generate the kallisto reference. Reads were pseudoaligned using the ‘kallisto bus’ command with the chemistry set to ‘VISIUM’...
A combination of a potentially very strong splice acceptor and an effective polyadenylation signal assures the complete disruption of the function of trapped ... I Yasumasa,L Philip - 《Nucleic Acids Research》 被引量: 163发表: 2000年 Compensation for a gene trap mutation in the murine microtubul...
Two canonical hexanucleotide polyadenylation signal sequences, AAUAAA or AUUAAA38,39, which were mostly identified 10–30 bases upstream of the cleavage/polyadenylation site, were less used in coding sequences (CDSs) (Fig. 1c). This was similar to the results of a previous study13, which ...