Bringing the two signals closer together resulted in decreased signal efficiency; with a separation of 7 or 9 bp, no tk mRNA polyadenylated within the normal region was produced. Altering the sequences between these two elements without changing the distance had small effects on processing ...
The recognition step is governed by at least two sequences: the polyadenylation signal (usually AAUAAA, but see below), and the U-rich or GU-rich downstream sequence element. The polyadenylation signal is found about 12–30 nucleotides upstream of the cleavage site, and the downstream sequence ...
For kallisto, a transcriptomic reference was also generated using the GRCm39 reference sequence and GENCODE M28 annotations. The default k-mer length of 31 was used to generate the kallisto reference. Reads were pseudoaligned using the ‘kallisto bus’ command with the chemistry set to ‘VISIUM’...
Alpha-thalassaemia caused by a polyadenylation signal mutation. Most eukaryotic messenger RNAs have the sequence AAUAAA 11–30 nucleotides from the 3′-terminal poly(A) tract 1,2 . Since this is the only significant sequence homology in the 3′ non-coding region it has been suggested that it ...
Overlapping polyadenylation signal sequences have not been described previously. An instance of gene overlap has been found involving a shared single functional polyadenylation site. The genes involved are the human alpha/beta hydrolase domain containing gene 1 (ABHD1) and Sec12 genes. The nine exon...
To address this question, we have applied a heuristic filters method to removing potential internal priming events based on the arbitrary cutoff of the number of adenines in the genome sequences. However, this could lead to unavoidably excluding some true poly(A) sites or including some ...
(APA) is a widespread phenomenon, generating mRNAs with alternative 3′ ends. APA contributes to the complexity of the transcriptome by generating isoforms that differ either in their coding sequence or in their 3′ untranslated regions (UTRs), thereby potentially regulating the function, stability,...
Polyadenylation signal sequenceIn studies regarding both gene therapy and gene function, transgene expression by plasmid vectors benefits from the use of ... Zhi-Li Xu and Hiroyuki Mizuguchi and Akiko Ishii-Watabe and Eriko Uchida and Tadanori Mayumi and Takao Hayakawa - 《Gene》 被引量: 334发表...
By means of specific binding proteins, cap and poly(A) tail function synergistically in translation, and during mRNA decay they are the first targets to be attacked by hydrolytic enzymes before the coding sequence is degraded. 3′ end processing leading to polyadenylated RNA consists of two ...
A G/U-rich region is usually situated downstream of the consensus signal sequence. There is evidence to suggest that this G/U region is involved with cleavage specificity, yet mutation of the G/U-rich tracts appears to affect the polyadenylation process only weakly (Zarkower and Wickens, 1988...