Using expression vectors constructed with genomic DNA segments composed of the μ m polyadenylation signal region, we analyzed poly(A) site utilization and termination of transcription in stably transfected myeloma cells and in murine fibroblast L cells. We found that the gene segment containing the ...
In contrast, a more refined series of mutations, in which clusters of 3 bases were replaced, failed to cause loss of function. We conclude that the downstream element of the mouse beta globin poly(A) signal is bipartite in structure, and that portions of its sequence are functionally ...
Class V (32% of the total) contained no known poly (A) signal. Analysis of the relative frequency of sequences between 1 and 60 nucleotides upstream from the poly (A) tail was determined for each cDNA sequence. Overall, the 60... AJ Wood,RJ Duff,Q Zeng,... - 《Bryologist》 被引...
Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full-...
(NEB). The primer sequences used were as follows: the putative STAT3-binding site region (PBR) of human FGFBP1, forward: 5′-CAGGAAGGGGAAGGGCCACAAG-3′; reverse: 5′-TGATCCTTGGGAGGCAGGCAGATT-3′; a nonspecific STAT3-binding region (NBR) of human FGFBP1, forward: 5′-GCCCACTATGA...
Cells lacking ZNF598 activity or containing ubiquitination-resistant eS10 ribosomes failed to stall efficiently on poly(A) sequences. In the absence of stalling, read-through of poly(A) produces a poly-lysine tag, which might alter the localization and solubility of the associated protein. Thus...
First, we show that iso-ADP-ribose (iso-ADPr), the smallest internal poly(ADP-ribose) (PAR) structural unit, binds between the WWE and RING domains of RNF146 and functions as an allosteric signal that switches the RING domain from a catalytically inactive state to an active one. In the...
Mucopolysaccharidosis IVA (MPS IVA; Morquio A syndrome) is a lysosomal storage disease caused by deficiency of N-acetylgalactosamine-6-sulfatase (GALNS) and transmitted as an autosomal recessive trait. This is the first systematic mutation screen in Chinese MPS IVA patients. Mutation detections in...
3). The lower left (LL) quadrant represents cells with a low fluorescence signal for RBITC at baseline and shifts to the lower right (LR) quadrant reflect an increase of RBITC fluorescence intensity indicative of NPs uptake. All cells, either infected or non-infected, showed a shift from ...
FCS quantifies fluctuations in the fluorescence intensity, as labeled molecules diffuse through a small excitation volume. The analysis of the fluorescent signal using an autocorrelation function (Equation 2) yields the diffusion coefficient of the macromolecule according to Ref. 24, (Eq. 2)G(τ)=...