SV40 early enhancer/promoter 7-418 Multiple cloning site 421-480 Chimeric intron 546-682 T7 RNA polymerase transcription(-17 to +2) 726-744 T7 RNA polymerase transcription initiation site 743 Rluc reporter gene 754-1689 SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding ...
常见的PAS tk polyA, SV40 late and early polyA or BGH polyA mammalian terminators (SV40, hGH, BGH, and rbGlob) SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是...
SV40 early enhancer/promoter7-418 Multiple cloning site421-480 Chimeric intron546-682 T7 RNA polymerase transcription(-17 to +2)726-744 T7 RNA polymerase transcription initiation site743 Rluc reporter gene754-1689 SV40 late polyadenylation signal1731-1932 ...
SV40 early enhancer/promoter 7-418 Multiple cloning site 421-480 Chimeric intron 546-682 T7 RNA polymerase transcription(-17 to +2) 726-744 T7 RNA polymerase transcription initiation site 743 Rluc reporter gene 754-1689 SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region...
多腺苷化信号又称PolyA信号(Polyadenylationsignal),AAUAAA(在基因组序列中表示为AAl’AAA)是其主要形式,参与真核mRNA3’端形成的重要过程。己知Alu序列和PolyA信号与多种生物学现象关联,如Alu的插入或缺失可引起肿瘤、染色体重组等;而一大类导致地中海贫血的血红蛋白病都归因于AAUA八A这个序列的点突变。本文中在...
SV40 early enhancer/promoter 7-418 Multiple cloning site 421-480 Chimeric intron 546-682 T7 RNA polymerase transcription(-17 to +2) 726-744 T7 RNA polymerase transcription initiation site 743 Rluc reporter gene 754-1689 SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) ...
SV40 early enhancer/promoter7-418 Multiple cloning site426-480 Chimeric intron546-682 T7 RNA polymerase transcription (-17 to +2)726-744 T7 RNA polymerase transcription initiation site743 Gluc (MT) reporter gene754-1311 SV40 late polyadenylation signal1352-1533 ...
SV40 early enhancer/promoter 7-418 Chimeric intron 490-626 T7 RNA polymerase transcription(-17 to +2) 670-688 T7 RNA polymerase transcription initiation site 687 Rluc reporter gene 698-1633 Multiple cloning site 1635-1701 SV40 late polyadenylation signal 1781-1942 β-lactamase (Ampr)...
aThe HA gene was cloned and engineered to be under the control of the cytomegalovirus immediate early promoter, within the HVT genome. The SV40 polyadenylation signal was added to the 3' terminus of the HA gene. HA基因被克隆并且被设计是受巨细胞病毒直接早期促进者的控制,在HVT染色体之内。 SV40 ...
"Terminator" is intended to include whatever is required in such sequences, not necessarily limited to the polyadenylation. function. This definition is necessitated by the current state of knowledge in the art, wherein the relevance of sequences in addition to the polyadenylation signal is unclear...