SV40 early enhancer/promoter 7-418 Multiple cloning site 421-480 Chimeric intron 546-682 T7 RNA polymerase transcription(-17 to +2) 726-744 T7 RNA polymerase transcription initiation site 743 Rluc reporter gene 754-1689 SV40 late polyadenylation signal ...
常见的PAS tk polyA, SV40 late and early polyA or BGH polyA mammalian terminators (SV40, hGH, BGH, and rbGlob) SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是...
SV40 early enhancer/promoter7-418 Multiple cloning site421-480 Chimeric intron546-682 T7 RNA polymerase transcription(-17 to +2)726-744 T7 RNA polymerase transcription initiation site743 Rluc reporter gene754-1689 SV40 late polyadenylation signal1731-1932 ...
SV40 early enhancer/promoter7-418 Chimeric intron490-626 T7 RNA polymerase transcription(-17 to +2)670-688 T7 RNA polymerase transcription initiation site687 Rluc reporter gene698-1633 Multiple cloning site1635-1701 SV40 late polyadenylation signal1781-1942 ...
SV40 early enhancer/promoter7-418 Multiple cloning site421-480 Chimeric intron546-682 T7 RNA polymerase transcription(-17 to +2)726-744 T7 RNA polymerase transcription initiation site743 Rluc reporter gene754-1689 SV40 late polyadenylation signal1731-1932 ...
多腺苷化信号又称PolyA信号(Polyadenylationsignal), AAUAAA(在基因组序列中表示为AAl’AAA)是其主要形式, 参与真核mRNA 3’端形成的重要过程。己知Alu序列和PolyA 信号与多种生物学现象关联,如Alu的插入或缺失可引起肿 瘤、染色体重组等;而一大类导致地中海贫血的血红蛋白病 ...
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substr...
Early Polyadenylation Signals of Human Papillomavirus Type 31 Negatively Regulate Capsid Gene Expression site or the late SV40 signal, suggesting that alterations in the strength of the upstream poly(A) signal influence expression of viral replication factors... SS Terhune,WG Hubert,JT Thomas,... -...
The plasmid pW-t8 carrying a similar fragment of SV40 DNA as pW2-t, but lacking the processing and polyadenylation signal sequences, also immortalized RE cells. Surprisingly, the plasmid pD-t1 which contains neither the intact large-T nor the small-t function also immortalized RE cells. ...
The soluble neuropilin-1 (sNRP-1) gene employs an extremely short dual function polyadenylation (pA) signal/stop codon that is efficient for termination of gene transcription and translation in vivo. However, the functionality and usefulness of this signal in regard to other genes is unknown. Thi...