SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱如下: EcoRI XhoI SacI MluI HindIII EcoRV 411 TTTTGCAAAA AGCTCCTCGA GGAGCTCACG
SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多*位点的详细图谱如下: EcoRI XhoI SacI MluI HindIII EcoRV 411 TTTTGCAAAA AGCTCCTCGA GGAGCTCACG CGTAAGCTTG AATTCGATAT AAAACGTTTT TCGAGGAGCT CCTCGAGTGC GCAT...
tk polyA, SV40 late and early polyA or BGH polyA mammalian terminators (SV40, hGH, BGH, and rbGlob) SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是最早被认识...
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substr...
A thalassemia caused by a polyadenylation signal mutation. Nature This idea is strengthened by observations on the effect of deletion mutations in or around the AATAAA sequence on polyadenylation of late simian virus 40 (SV40) mRNA; removal of this sequence prevents poly(A) addition. Naturally .....
"Terminator" is intended to include whatever is required in such sequences, not necessarily limited to the polyadenylation. function. This definition is necessitated by the current state of knowledge in the art, wherein the relevance of sequences in addition to the polyadenylation signal is unclear...
aThe HA gene was cloned and engineered to be under the control of the cytomegalovirus immediate early promoter, within the HVT genome. The SV40 polyadenylation signal was added to the 3' terminus of the HA gene. HA基因被克隆并且被设计是受巨细胞病毒直接早期促进者的控制,在HVT染色体之内。 SV40 ...
The 5′ untranslated region does not show the extensive repeated sequences found in the similar region of SV40. The 3′ untranslated region contains the sequence 5′-AATAAA-3′, thought to represent a polyadenylation signal. As in the early region of polyoma, the extensive nucleotide and ...
SV40 late polyadenylation signal1781-1942 β-lactamase (Ampr) coding region2079-2494 pRL-SV40-C质粒(3775bp)的图谱如下: pRL-SV40-C的多*位点的详细图谱如下: EcoRI XhoISacIMluIHindIIIEcoRVSalI 1631TAATTCTAGGCTCGAGGAGCTCACGCGTAAGCTTGAATTCGATATCGTCG ...