tk polyA, SV40 late and early polyA or BGH polyA mammalian terminators (SV40, hGH, BGH, and rbGlob) SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是最早被认识...
SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多克隆位点的详细图谱如下: EcoRI XhoI SacI MluI HindIII EcoRV 411 TTTTGCAAAA AGCTCCTCGA GGAGCTCACG
SV40 late polyadenylation signal1781-1942 β-lactamase (Ampr) coding region2079-2494 pRL-SV40-C质粒(3775bp)的图谱如下: pRL-SV40-C的多*位点的详细图谱如下: EcoRI XhoISacIMluIHindIIIEcoRVSalI 1631TAATTCTAGGCTCGAGGAGCTCACGCGTAAGCTTGAATTCGATATCGTCG ...
We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substr...
Sequence reference points: SV40 enhancer and early promoter 7–418 Chimeric intron 486–622 T7 RNA polymerase promoter (–17 to +2) 666–684 T7 RNA polymerase transcription initiation site 683 Rluc reporter gene 694–1629 SV40 late polyadenylation signal 1671–1872 β-lactamase (Amp r ) coding...
Early Polyadenylation Signals of Human Papillomavirus Type 31 Negatively Regulate Capsid Gene Expression site or the late SV40 signal, suggesting that alterations in the strength of the upstream poly(A) signal influence expression of viral replication factors... SS Terhune,WG Hubert,JT Thomas,... -...
A G/U-rich region is usually situated downstream of the consensus signal sequence. There is evidence to suggest that this G/U region is involved with cleavage specificity, yet mutation of the G/U-rich tracts appears to affect the polyadenylation process only weakly (Zarkower and Wickens, 1988...
The mouse DNA fragment, which contains an intervening sequence and a poly(A) addition site, has been inserted in both possible orientations relative to the SV40 late region promoter. While the fragment is transcribed regardless of orientation, the RNA splice signal and poly(A) addition site are...
SV40 late polyadenylation signal 1731-1932 β-lactamase (Ampr) coding region 2079-2939 pRL-SV40-N质粒(3765bp)的图谱如下: pRL-SV40-N的多*位点的详细图谱如下: EcoRI XhoI SacI MluI HindIII EcoRV 411 TTTTGCAAAA AGCTCCTCGA GGAGCTCACG CGTAAGCTTG AATTCGATAT AAAACGTTTT TCGAGGAGCT CCTCGAGTGC GCAT...