sequences were stronger than those of their corresponding single sequences.Secloop (22 bp near the center in 2F2R) and Poly4 (30 bp in 2F2R 3' end) sequences did not activate GFP gene expression.The activating action of 30R-Poly4 sequence formed by ligating 30R with Poly4 by 9 bp ...
关键词:Alu;SV40PolyA;AATAAA信号;GFP;转录终止 TheeffectsofSV40PolyAsequenceanditsAATAAAsignalonup- streamGFPgeneexpressionandtranscriptiontermination LIShu-Ping 1,2 ,FENGJing-Jing 3 ,WANGHong-Gang 4 ,WANGXiu-Fang 1 ,LVZhan-Jun 1 1.DepartmentofGenetics,HebeiKeyLabofLaboratoryAnimal,HebeiMedical...
[ Abstract] Objective ToinvestigatetheregulatoryeffectsoftwokindsofSV40poly(A)signalsontheupstreamgeneexpressioninthreecelllines, soastoprovidetheoreticalevidenceforselectionofpoly(A)ofvectors.Methods AdualluciferasereportervectorDual-Lucwasconstructed, andtwoSV40poly(A)signalsequenceswereinsertedinthedownstreamof...
关键词:非编码序列;GFP;茎环结构;Alu;SV40PolyA Detectionandsequenceanalysisofanimperfectstem-loopstructure incisactivatinggeneelementfromSV40PolyA WANGHong-Gang 1,2 ,MAHuan 1 ,LIZhu 3 ,ZHANGBin 3 ,JINGXiang-Yang 1 , ZHANGYuan 1 ,LUZhan-Jun 1 1.DepartmentofGenetics,HebeiKeyLabofLaboratoryAnimal,...
Keywords: non-coding sequence; GFP; stem-loop; Alu; SV40PolyA 真核和原核生物基因组中均存在非编码序列,真核生物基因组中非编码序列较多,原核 生物基因组中非编码序列较少,如人基因组中大于 95% 的序列属于非编码序列,而大肠杆 菌基因组中非编码序列约占 11%。非编码序列中蕴藏着重要的有关遗传和发育的...
This idea is strengthened by observations on the effect of deletion mutations in or around the AATAAA sequence on polyadenylation of late simian virus 40 (SV40) mRNA; removal of this sequence prevents poly(A) addition. Naturally ... DR Higgs,SEY Goodbourn,J Lamb,... - 《Nature》 被引量:...
环结构均不利于活化基因，提示适当的不完整茎环结构与活化基因有关。关键词：非编码序列；GFP；茎环结构；Alu；SV40PolyA Detection and sequence analysis of an imperfect stem-loop structure in cis activating gene element from SV40PolyA WANG Hong-Gang1,2, MA Huan1, LI Zhu3, ZHANG Bin3, JING Xiang-...
A SERIES of recent reports have demonstrated the covalent association of long polyadenylic acid (poly A) sequences with mammalian cytoplasmic messenger RNA and with its putative precursor, the heterogeneous nuclear RNA. These studies indicate that the poly A sequences are attached to messenger RNA ...
在这两种情况下,突变 PAS 的亚克隆表明它失去了 poly(A) 形成功能。 尽管AAUAAA 序列被证明是 mRNA 3'-末端多聚腺苷酸化所必需的,但其他序列元素被证明是完全重建功能性 PAS 所必需的。 特别是,在紧邻基因 3' 侧翼区域中的 mRNA 3' 末端之后存在的富含 GU 的序列(或下游序列元件,DS,downstream sequence el...
(A) of vectors.Methods A dual luciferase reporter vector Dual-Luc was constructed,and two SV40 poly(A) signal sequences were inserted in the downstream of the R-Luc gene separately.Then the two diverse dual luciferase reporter gene vectors Dual-Luc2 and Dual-Luc3 were transfected into 293,...