Considerable difficulties are involved in the study of immunoglobulins. Data on N-terminal amino acids, which have been useful for an understanding of the structure of many proteins, have not been of any essential help here. As mentioned earlier (cf. Chapter III), only one N-terminal amino ...
However, rational design of antibodies is hindered by reliance on experimental methods for determining antibody structures. Here, we present DeepAb, a deep learning method for predicting accurate antibody FV structures from sequence. We evaluate DeepAb on a set of structurally diverse, therapeutically ...
The negative fraction was then enriched for CD1c þ dendritic cells by labelling them with anti-CD1c biotinylated antibodies (1:15 dilution), followed by anti-biotin microbeads (Myltenyi Biotec). CD1c þ DCs were cultured immediately after purification in RPMI containing 10% fetal calf serum...
Briefly, 293T cells were transfected with relevant expression plasmids and lysates were subjected to immunopre- cipitation with indicated antibodies followed by SDS-PAGE electrophoresis and immunoblotting. Shown are representative gels. All experiments were performed at least 3 times to ensure ...
To obtain an insight into its molecular identity, we generated several hundred monoclonal antibodies that recognized rhesus PBMCs and tested their abilities to block the presentation of C14nef5 to 2N5.1. Two blocking monoclonal antibodies, MB217 and MB226, were isolated (Supplementary Fig. 1a) ...
Article: The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus
Understand how the characterization of antibody structure led to the comprehension of antibody function. 2. Distinguish between the overall structure and the fine struc- ture of antibodies. 3. Describe the variable and constant regions of an antibody's light and heavy chains. 4. Explain the ...
Interestingly, some monoclonal antibodies bind exclu- sively to the dimeric M2e, while others can bind to both monomeric and dimeric forms of M2e [20]. Such interac- tions of M2e peptide with antibody and its dimerization capability in the context of inhibiting virulence have not been studied ...
The His-gankyrin (either wild-type or mutant) and S6 ATPase (either S6-C and S6-A) that coprecipitated with GST-pRb was detected by western blotting with antibodies against the His-tag (Cell Signaling, #27E8) and against S6 ATPase (BIOMOL, #PW8175), followed by a horseradish peroxidase...
Antibodies are currently the most important class of biotherapeutics and are used to treat numerous diseases. Recent advances in computational methods are ushering in a new era of antibody design, driven in part by accurate structure prediction. Previously, structure-based antibody design has been limi...