Here, we describe a simple strand-specific RNA-seq protocol for identifying and quantifying RNA species within a sample compatible with both SOLiD and Illumina Genome Analyzer second-generation DNA sequencers. Although several approaches exist for generating shotgun libraries of the transcriptome, the ...
Stranded, high-quality RNA-seq libraries for transcriptome analysis Only 100–5000 ng of total RNA or 1–100 ng of poly-A+ enriched RNA required Includes QIAseq Beads and sample adapter plates Compatible with fresh, as well as FFPE samples Includes access to the RNA-seq Analysis Portal for ...
SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian: improved ease of use and sequencing performance for whole transcriptome analysis of high-quality or degraded samples A versatile solution for low-input total RNA-seqKit accommodates 250 pg–10 ng i...
We performed RNA-seq of tumor samples from cervical cancer patients in local cohort and also assessed the RNA-seq and clinical data derived from public datasets. By using single sample Gene Set Enrichment Analysis (ssGSEA) and univariate Cox analysis, patients were stratified into distinct dsRBP ...
High Performance Liquid Chromatographic analysis of the Biological Response Modifier Synthetic Double-Stranded RNA (dsRNA, Ampligen) A high performance liquid chromatographic method was developed for analysis of the antitumor agent synthetic, double-stranded RNA (dsRNA, Ampligen) in plasma. In this procedu...
numbers of the ERCC RNA spike-in.gLinear correlation between the number of UMIs in the LAST-seq data and the input copy numbers of the ERCC RNA spike-in.hCoefficient of determination (R2) of the linear regression in (f) and (g), from 10 samples. The statistical analysis was performed ...
RNA-Seq on Illumina platforms for mammalian samples Coding and non-coding transcript information Additional Product Information Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to ...
De novo assembly and analysis of RNA-seq data. Nat Methods. 2010;7:909–12. Article CAS PubMed Google Scholar Hoang ML, et al. Genome-wide quantification of rare somatic mutations in normal human tissues using massively parallel sequencing. Proc Natl Acad Sci U S A. 2016;113:9846–51...
My thesis work shows that SID-1 is selectively and reversibly activated by and transports dsRNA of any length, including short-interfering RNA (siRNA). Genetic analysis in shows that is required for both import and export of silencing signals, indicating that dsRNA SID-1 is bidirectional. ...
Discovery across both coding and multiple forms of noncoding RNA High-quality whole-transcriptome analysis with precise strand information These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mous...