Even without a genome sequence, RNA-seq can in principal be used for building transcript contigs akin to UniGene assembled transcripts from the 1990s. With each of these applications in mind we discuss aspects of experimental design, such as read length, depth of sequencing, replicates, and ...
Double-stranded RNA(dsRNA)were extracted from leaf tissues of radish(Raphanus sativus-root 'Yidianhong')grown in Hangzhou China.Complementary DNAs corresponding to the dsRNA segments were obtained by using a modified single primer amplification technique(SPAT),and followed by cloning and sequencing.The...
SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian: improved ease of use and sequencing performance for whole transcriptome analysis of high-quality or degraded samples A versatile solution for low-input total RNA-seqKit accommodates 250 pg–10 ng i...
RNA-seq methods are widely utilized for transcriptomic profiling of biological samples. However, there are known caveats of this technology which can skew the gene expression estimates. Specifically, if the library preparation protocol does not retain RNA strand information then some genes can be ...
Northern hybridization analyses using an RNA probe corresponding to the 3'-terminal region of apple stem grooving virus (ASGV) indicated that all dsRNA preparations from tissues infected with 10 ASGV isolates contained three virus-specific dsRNAs (6.5, 2.0 and 1.0 kbp). Using additional RNA probes...
numbers of the ERCC RNA spike-in.gLinear correlation between the number of UMIs in the LAST-seq data and the input copy numbers of the ERCC RNA spike-in.hCoefficient of determination (R2) of the linear regression in (f) and (g), from 10 samples. The statistical analysis was performed ...
We performed RNA-seq of tumor samples from cervical cancer patients in local cohort and also assessed the RNA-seq and clinical data derived from public datasets. By using single sample Gene Set Enrichment Analysis (ssGSEA) and univariate Cox analysis, patients were stratified into distinct dsRBP ...
RNA interference (RNAi) based methods are being developed for pest management. A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use
Double-Stranded RNA-Binding Domain (dsRBD) A short sequence motif consisting of approximately 70 amino acid residues has been identified in a large number of functionally diverse proteins including Escherichia coli RNase III, Drosophila Staufen protein, double-stranded RNA (dsRNA)-dependent adenosine dea...
3’ end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character-RNA-Seq analyses Nucleic Acids Res., 46 (2018), pp. 9253-9263 Google Scholar 20 X. Mu, E. Greenwald, S. Ahmad, S. Hur An origin of the immunogenicity of in vitro transcribed RNA Nucl...