Comparison of stranded and unstranded RNA-seq library methods and their influence on interpretation and analysis.Malachi, GriffithJason, R. WalkerNicholas, C. SpiesBenjamin, J. AinscoughObi L., Griffith
(1)oligo-dT富集:我们一般说的RNA-seq是指mRNA,但是我们提出来的总RNA有接近90%全部是rRNA,我们只想得到mRNA,利用真核生物mRNA一般都带有polyA的尾巴这一性质我们设计oligo-dT去与这个polyA的尾巴进行杂交,就能把我们想要的mRNA给富集出来。 为什么只有mRNA被加尾? 在真核生物中,mRNA由RNA聚合酶II转录,而rRNA大部...
Validated lncRNA from blood stranded RNA-Seq that overlap with class 3 un-stranded lncRNA.Lambros, T. KoufariotisYiPing, Phoebe ChenAmanda, ChamberlainChristy, Vander JagtBen, J. Hayes