(1)oligo-dT富集:我们一般说的RNA-seq是指mRNA,但是我们提出来的总RNA有接近90%全部是rRNA,我们只想得到mRNA,利用真核生物mRNA一般都带有polyA的尾巴这一性质我们设计oligo-dT去与这个polyA的尾巴进行杂交,就能把我们想要的mRNA给富集出来。 为什么只有mRNA被加尾? 在真核生物中,mRNA由RNA聚合酶II转录,而rRNA大部...
RNA-seq methods are widely utilized for transcriptomic profiling of biological samples. However, there are known caveats of this technology which can skew the gene expression estimates. Specifically, if the library preparation protocol does not retain RNA strand information then some genes can be ...
Comparison of stranded and unstranded RNA-seq library methods and their influence on interpretation and analysis.Malachi, GriffithJason, R. WalkerNicholas, C. SpiesBenjamin, J. AinscoughObi L., Griffith
Validated lncRNA from blood stranded RNA-Seq that overlap with class 3 un-stranded lncRNA.Lambros, T. KoufariotisYiPing, Phoebe ChenAmanda, ChamberlainChristy, Vander JagtBen, J. Hayes