RNA-SeqPNPaseRibonucleaseRNA maturationtRNAOrganellar gene expression incorporates ribonucleases as indispensable participants. Here, we explored the capacity of strand-specific RNA sequencing (RNA-Seq) as a tool to analyze chloroplast ribonuclease functions using the 3′→5′ exoribonuclease polynucleotide ...
对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: --rf Assumes a stranded library fr-firststrand. --fr Assumes a stranded library fr-secondstrand. kallisto: --fr-stranded runs kallisto in strand specific mode, only fragments where the first ...
与RNA序列的一致性:除了T被U取代外,Sense Strand的碱基序列与RNA序列基本一致。这种一致性使得研究人员可以通过分析Sense Strand的序列来预测和了解RNA的序列,进而推断蛋白质的结构和功能。 三、在链特异性测序中的应用 在链特异性测序(Strand-specific RNA-Seq)技术中,Sense Strand的概念得到...
High-throughput illumina strand-specific RNA sequencing library preparation. Conventional Illumina RNA-Seq does not have the resolution to decode the complex eukaryote transcriptome due to the lack of RNA polarity information. Strand-specific RNA sequencing (ssRNA-Seq) can overcome these limitations and ...
All three RNA-seq methods used in this study are strand-specific. While Illumina TruSeq library kit uses dUTP labeling to degrade the second strand, Swift and Swift Rapid RNA kits maintain strand-specificity by making only one functional strand which is immediately ligated with a 3’ truncated ...
链特异性主要对环形和lnc项目，区别在于链特异性文库构建时使用dUtp合成二链，扩增前使用User酶消化使得...
The SENSE mRNA-seq library preparation kit generates ready-to-sequence libraries from low amounts of total RNA within 4 h. SENSE libraries are significantly more strand specific (>99.9%) than leading alternatives and are virtually free of rRNA contami
Then, we applied strand-specific RNA-seq to investigate the effects of this microbiota on the global gene expression of EHEC O2621765 and O157EDL933 strains by comparison with their behavior in beef meat without microbiota. In strain O2621765, the expression of genes connected with nitrate ...
Strand-specific ChIP-seq recapitulated the ssDNA-binding nature of RPA and the Rad51 recombinase, but also visualized the dsDNA binding of Rad51 during homology search and the 9-1-1 signaling platform. Importantly, we found that all histones, including histone variants and DNA damage-specific ...
First strand cDNA synthesized by random hexamer priming on RNA showed consistent position and nucleotide-specific mismatch errors in the first seven nucleotides. The mismatch errors found in both datasets are consistent in distribution and thermodynamically stable mismatches are more common. This strongly ...