FRT-seq: amplification-free, strand-specific transcriptome sequencing. Nat. Methods - Mamanova, Andrews, et al. - 2010 () Citation Context ...). One common feature of all these reports was the usage of PCR amplification as a part of the RNA preparation for sequencing, which has the ...
对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: 代码语言:javascript 复制 --rf Assumes a stranded library fr-firststrand.--fr Assumes a stranded library fr-secondstrand. kallisto: 代码语言:javascript 复制 --fr-stranded runs kallistoinstrand spec...
对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: --rf Assumes a stranded library fr-firststrand. --fr Assumes a stranded library fr-secondstrand. kallisto: --fr-stranded runs kallisto in strand specific mode, only fragments where the first ...
Strand-Specific RNA-Seq Reveals Ordered Patterns of Sense and Antisense Transcription in Bacillus anthracis 来自 plosone.org 喜欢 0 阅读量: 62 作者:KD Passalacqua,A Varadarajan,C Weist,BD Ondov,B Byrd,TD Read,NH Bergman 摘要: Although genome-wide transcriptional analysis has been used for many...
Here, we present a strand-specific RNA-seq dataset for both coding and lncRNA profiling in myocardial tissues from 28 HCM patients and 9 healthy donors. This dataset constitutes a valuable resource for the community to examine the dysregulated coding and lncRNA genes in HCM versus normal ...
High-throughput illumina strand-specific RNA sequencing library preparation. Conventional Illumina RNA-Seq does not have the resolution to decode the complex eukaryote transcriptome due to the lack of RNA polarity information. Strand-specific RNA sequencing (ssRNA-Seq) can overcome these limitations and ...
Example data tables and corresponding plots are shown below for different data configurations, including strand-specific data, non-strand-specific data, and then the case where strand-specific data were assembled as non-strand-specific (oops!). Non-strand-specific RNA-Seq data Example data table:...
链特异性主要对环形和lnc项目，区别在于链特异性文库构建时使用dUtp合成二链，扩增前使用User酶消化使得...
These scripts are easily customized to meet specific research needs and improve on existing informatics and statistical treatments of RNA-seq data. In particular, we apply significance tests for determining differential gene expression and intron retention events. To demonstrate the potential of both the...
The SENSE mRNA-seq library preparation kit generates ready-to-sequence libraries from low amounts of total RNA within 4 h. SENSE libraries are significantly more strand specific (>99.9%) than leading alternatives and are virtually free of rRNA contami