The strand-specific protocol presented here uses RNA fragmentation to generate short RNA fragments that are then converted to cDNA using an anchored random primer and a template switch primer. We discuss the application of the technology, and see great scope for its use in both model organisms ...
对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: --rf Assumes a stranded library fr-firststrand. --fr Assumes a stranded library fr-secondstrand. kallisto: --fr-stranded runs kallisto in strand specific mode, only fragments where the first ...
High-throughput illumina strand-specific RNA sequencing library preparation. Conventional Illumina RNA-Seq does not have the resolution to decode the complex eukaryote transcriptome due to the lack of RNA polarity information. Strand-specific RNA sequencing (ssRNA-Seq) can overcome these limitations and ...
链特异性主要对环形和lnc项目，区别在于链特异性文库构建时使用dUtp合成二链，扩增前使用User酶消化使得...
All three RNA-seq methods used in this study are strand-specific. While Illumina TruSeq library kit uses dUTP labeling to degrade the second strand, Swift and Swift Rapid RNA kits maintain strand-specificity by making only one functional strand which is immediately ligated with a 3’ truncated ...
Then, we applied strand-specific RNA-seq to investigate the effects of this microbiota on the global gene expression of EHEC O2621765 and O157EDL933 strains by comparison with their behavior in beef meat without microbiota. In strain O2621765, the expression of genes connected with nitrate ...
The SENSE mRNA-seq library preparation kit generates ready-to-sequence libraries from low amounts of total RNA within 4 h. SENSE libraries are significantly more strand specific (>99.9%) than leading alternatives and are virtually free of rRNA contami
Our data show that strand-specific ChIP-seq allows us to discriminate protein binding to ssDNA or dsDNA at localized DSBs and define ssDNA and dsDNA domains. In the case of nucleosomes, recent data have been taken as an indication that nucleosomes could stay on ssDNA during resection or re-...
Such information could be used, for example, to understand what type of structural motifs are common in a specific set of RNA molecules; to estimate the accuracy of RNA secondary structure computational prediction methods; or to improve current thermodynamic models for RNA secondary structure ...
YeaCellTM1st Strand Synthesis kit for Single Cell 3ʹ RNA-seq利用Oligo (dT)18及TSO Primer引物对单细胞转录本基因进行逆转录,适用于微流控的微量体系。其产物可以经过通用引物进行PCR反应富集cDNA,然后使用翌圣的酶切建库试剂盒,构建测序文库。本产品采用了在M-MLV(RNase H-)Reverse Transcriptase基础上经过多...