Stern, 2013 Strand-specific RNA sequencing uncovers chloroplast ribonuclease functions. FEBS letters 587 (18):3096-3101.Castandet, B., Hotto, A.M., Fei, Z., and Stern, D.B. (2013). Strand- specific RNA sequencing uncovers chloroplast ribonuclease func- tions. FEBS Lett. 587: 3096-3101....
对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: --rf Assumes a stranded library fr-firststrand. --fr Assumes a stranded library fr-secondstrand. kallisto: --fr-stranded runs kallisto in strand specific mode, only fragments where the first ...
RSeQC判断链特异性(strand-specific) 对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: 代码语言:javascript 代码运行次数: --rf Assumes a stranded library fr-firststrand.--fr Assumes a stranded library fr kallisto: 代码语言:javascript 代码运行次数:0...
在看一些关于分析strand-specific RNA sequencing软件参数的时候，看到 “Indicate if strand-specific read...
High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract ...
Despite the recent precipitous decline in the cost of genome sequencing, library preparation for RNA-seq is still laborious and expensive for applications such as high throughput screening. Limited availability of RNA generated by some experimental workf
Then, we applied strand-specific RNA-seq to investigate the effects of this microbiota on the global gene expression of EHEC O2621765 and O157EDL933 strains by comparison with their behavior in beef meat without microbiota. In strain O2621765, the expression of genes connected with nitrate ...
Thus, in this system, mutational strand bias is independent of the selection for phenotype. The results are consistent with the model of preferential repair of the transcribed strand as proposed by others. The involvement of RNA polymerase in the selective repair recruitment is discussed. 展开 ...
lncRNA strand-specific transcriptome smRNA WES WGS或组装 QC non-strand specific transcriptome WGBS Demo数据 建库试剂盒名称平台读长样本样本信息下载 MGIEasy Fast RNA 文库制备试剂套装 MGISEQ-2000 PE150 UHRR 查看详细 MGIEasy Fast RNA 文库制备试剂套装 MGISEQ-2000 PE150 UHRR 查看详细 MGIEasy Fast RNA...
transcriptome lncRNA strand-specific transcriptome smRNA WES WGS或组装 QC non-strand specific transcriptome WGBS Demo数据 建库试剂盒名称平台读长样本样本信息下载 MGIEasy Fast RNA 文库制备试剂套装 DNBSEQ-T7 PE150 UHRR 查看详细 MGIEasy Fast RNA 文库制备试剂套装 DNBSEQ-T7 PE150 UHRR 查看详细 公司...