Stern, 2013 Strand-specific RNA sequencing uncovers chloroplast ribonuclease functions. FEBS letters 587 (18):3096-3101.Castandet, B., Hotto, A.M., Fei, Z., and Stern, D.B. (2013). Strand- specific RNA sequencing uncovers chloroplast ribonuclease func- tions. FEBS Lett. 587: 3096-3101....
Conventional Illumina RNA-Seq does not have the resolution to decode the complex eukaryote transcriptome due to the lack of RNA polarity information. Strand-specific RNA sequencing (ssRNA-Seq) can overcome these limitations and as such i... S Zhong,JG Joung,Y Zheng,... - 《Cold Spring Harbor...
Despite the recent precipitous decline in the cost of genome sequencing, library preparation for RNA-seq is still laborious and expensive for applications such as high throughput screening. Limited availability of RNA generated by some experimental workf
在看一些关于分析strand-specific RNA sequencing软件参数的时候，看到 “Indicate if strand-specific read...
Several library preparation methods for strand-specific sequencing of RNA (ssRNA-seq) have been developed in the recent past3–8. However, a majority of these methods involve the synthesis of second-strand cDNA after reverse transcription (RT) that can entail multiple artefacts, including the loss...
This constraint also influenced our sequencing strategy, sequencing mode (paired-end sequencing), library-type (strand-specific cDNA library) and choice of the read length (125 bp). The paired-end strand-specific RNA-Seq with a read length of 125 bp allows for higher levels of specific alignme...
The abundance of Arabidopsis strand-specific modifications points to their importance, perhaps as epigenetic signals that mark the heterochromatic regions that confer centromere activity. 展开 关键词: heterochromatin centromere methylcytosine DNA methylation ...
Here, we use site-specific induction of DSBs and chromatin immunoprecipitation followed by strand-specific sequencing to analyze in vivo binding of key DSB repair and signaling proteins to either the ssDNA or dsDNA domain. In the case of nucleosomes, we show that recently proposed ssDNA nucleosome...
Under High Mg2+ conditions, however, the half-length fragments are seen with Lower (5′-terminated) strand labeled substrates (lanes 2 and 3) but not with Upper (3′-terminated) strand labeled substrates (lanes 9 and 10), confirming the strand-specific degradation previously reported under ...
应用范围广:可用于使用Random Primer、Oligo dT Primer以及Specific Primer的各种逆转录情况 实验案例 1.不同长度RNA模板的逆转录能力 以1μg HeLa细胞Total RNA为模板,Oligo(dT)23VN为引物按照说明书体系和程序进行逆转录,取1μL cDNA产物模板,用PfuMax HiFi DNA Polymerase进行扩增,可见R413产品可完整逆转录长达...