STING的激活,会招募TANK结合激酶1 (TBK1),它能磷酸化干扰素调节因子3 (IRF3)和核因子-kappa B (NF-κB)等转录因子,诱导I型干扰素(IFN)和促炎细胞因子包括肿瘤坏死因子-α (TNF-α)、白细胞介素1β (IL-1β)和IL-6的产生。越...
活化的 STING 可迁移并激活 TBK1,诱导 IRF3 和 NF-κB 的磷酸化,从而触发促炎细胞因子的转录。与此相一致,scRNA-seq 数据也显示转录因子 Nfkb1 和 Irf3 的调节子活性在 Gsdmd-/-ApoE-/- 小鼠中降低,这些降低主要发生在 M1 巨噬细胞中。因此,作者推测 GSDMD 在氧化-LDL 诱导的 MPMs 中通过线粒体穿孔调节...
磷酸化的IRF3形成二聚体,转移至细胞核,诱导下游I型干扰素(type I interferon,IFN-Ⅰ)和干扰素刺激基因(interferon stimulatory genes,ISGs)的转录表达,启动先天性免疫反应[13]。此外,STING还可激活经典的NF-κB(nuclear factor kappa-B,...
IRF3-JUN转录因子复合物有利于ACOD1上调 作者接下来试图确定负责 STING1 依赖性 ACOD1 上调的转录因子。作者专注于干扰素调节因子 3 (IRF3) 和核因子-kappa B (NF-κB),这两种调节 STING1 依赖性细胞因子表达的众所周知的转录因子 (DNA sensing by the cGAS-STING pathway in health and disease. Nat. Rev...
Furthermore, the same phenomenon was also observed in Irf3−/− macrophages (Supplementary Fig. 1B) and cgas−/− L929 cells (Supplementary Fig. 1C), which means the cGAS-STING pathway and type I IFNs were not indispensable for the regulation of UFL1 expression. Previous reports ...
转移至高尔基体后, 多聚化的 STING 通过 CTT 招募 TBK1 并使 STING 和 IRF‑3 被 TBK1 磷酸化,磷酸化的 IRF3 入核后调控 I 型 IFNs 及 ISGs 的表达[10].此外,TBK1 也可以激活 IκB 激酶(Iκκ),使核转录因子 κB(nuclear factor‑kappa B, NF-κB)的抑制因子 IκB 磷酸化,磷酸化的 IκB ...
Most prominently, the outcome of cGAS signalling is the activation of inflammatory transcription through interferon regulatory factor 3 (IRF3) and nuclear factor kappa B (NF-kB). In addition, the cGAS-STING pathway can lead to the direct modulation of cellular processes independently of ...
TBK1 also phosphorylates Iκβα, an inhibitor of the transcription factor NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells), marking it for proteasomal degradation; Iκβα degradation releases NF-κB, which translocates together with IRF3 into the nucleus, providing a ...
NEMO-IKK beta Are Essential for IRF3 and NF-kappa B Activation in the cGAS-STING Pathway NEMO-IKKβ are essential for IRF3 and NF-κB activation in the cGAS-STING pathway. J Immunol (2017) 199(9):3222-33. doi:10.4049/jimmunol.1700699Fang R, Wang CG, Jiang QF, Lv MZ, Gao PF,...
(interferon regulatory factor 3, IRF3)核转位, 产生Ⅰ型干扰素(interferons, IFNs); 另一方面, STING会活化IκB激酶(inhibitor of nuclear factor kappa-B kinase, IKK), IKK将细胞内NF-κB-IκB复合物的IκB亚基调节位点的丝氨酸磷酸化, 进而被蛋白酶降解, 从而释放NF-κB二聚体进入细胞核, 与有NF-κB...