DNA isolation is a crucial first step in the PCR workflow, whether you are isolating genomic DNA (gDNA) or bacterial plasmids. Selecting nucleic acid purification products that are optimized to provide maximum yield, purity, and integrity from virtually any sample type and application is important ...
Cystobacter velatus Cbv34 and Myxococcus fulvus SBMx122 are native cystobactamid producer strains and were used for the isolation of genomic DNA or controls in production screening experiments, respectively. Cultivation was done in CTT medium (10 g L−1 casitone, 1.21 gL−1 TRIS, ...
the first step is to extract genomic DNA (please see theprotocol for DNA extraction). If the first step to generate a cDNA library relies on mRNA extraction, please see theprotocol for RNA extraction. Afterwards, the mRNA is converted to cDNA through the catalytic activity of reverse transcript...
Step 1- Nucleic Acid Extraction and Isolation Nucleic acid extraction and isolation is a vital first step in next generation sequencing. This is regardless of whether you are sequencing total RNA, genomic DNA, or various RNA types. The extraction method that’s used will depend ...
The goal of this dissertation is to describe the isolation of genetic markers and genomic clones that are linked to Ma1 as steps toward map-based cloning of the gene. AFLP markers were first optimized for amplifying sorghum DNA. A sorghum genetic map using 245 AFLPs was constructed covering ...
Whole-genome sequencing, or WGS, is performed to sequence the entire genome of an organism using the total genomic DNA. WGS data of a sample is then compared to a reference sample or control—for instance, comparison between...
The single positive-sense genomic RNA of ssRNA phages, which is protected by a capsid shell, is delivered into the host via the retraction of the host pili. Structures involved in ssRNA phage infection cycle are essential for understanding the underlying mechanisms that can be used to engineer ...
The DNA sequences that encode the two-subunit transketolase Rif15, and the separated subunits Rif15a and Rif15b were amplified from the genomic DNA ofAmycolatopsis mediterraneiU32 (A. mediterraneiU32 was deposited in Institute of Microbiology, Chinese Academy of Sciences designated as CGMCC4.5720) ...
protein isolation. 5. Add 0.4 ml isopropanol per 1 ml TRIzol used for original RNA isolation. Mix and incubate for 5 min. at RT. 6. Spin samples at 12,000 x g for 15 min. at 4 C. 7. Remove supernatant, keep pellet with DNA. Add 0.5 ml of ethanol 70% per 1 ml TRIzol used ...
ThelacZgene was amplified from the genomic DNA of strain MG1655 with primerslacZ-for-KpnI andlacZ-rev-BglII and ligated into plasmid pVanR carrying gene encoding the VanR mutant HyT12, after digestion withKpnI andBglII. Then the fragment containing PvanABK’controlledlacZ(PvanABK’-lacZ) an...