Additionally, genomic sequences around genes are captured including regulatory elements in introns and up- and downstream flanks. High similarity of the results obtained by a de novo assembly approach with a reference based mapping in rice supports the applicability for studying and understanding the ...
The described setup provides a fully integrated solution for T-cell screening, isolation and recovery on the single-cell level and can be linked to downstream genomic analysis. The ability to isolate a single T cell based on its specificity for an antigen and the ability to sequence its TCR ...
Substrates commonly used for restriction enzyme digestion include phage DNA, plasmid DNA, genomic DNA, PCR products and double-stranded oligonucleotides. The concentration of the DNA sample can influence the success of a restriction digestion. Viscous DNA solutions, resulting from large amounts of DNA...
When the focus of the study is on the bacterial community, the two main approaches in this framework are shotgun metagenomics, based on the sequencing of the whole genomic content of the community [2], and 16S rRNA gene sequencing (16S rDNA-Seq), a targeted sequencing of the gene that ...
and yield variability across samples, resulting in inaccurate data analysis and low reproducibility. Furthermore, if you determine that you have genomic DNA contamination, you may have to follow up your RNA extraction with a DNase I digestion, which can add additional time to your overall workflow...
The process of DNA replication contains many steps. The first step is the necessity for the double-stranded DNA molecule to be opened up into single strands by the enzyme DNA helicase, which makes a replication fork. The second step is the addition ...
One of its important functions, just like other oncoviruses, is to suppress tumor suppressor proteins like p53 and pRb to enhance genomic instability and induce uncontrollable cell proliferation [77]. It also regulates cyclin-dependent inhibitor p21- expression as it keeps a check on unwanted cell...
& Dong, X. A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathog. 2, e123 (2006). Article CAS PubMed PubMed Central Google Scholar Sugano, S. et al. Role of OsNPR1 in rice defense program as revealed by ...
After one week cells were subjected to cell sorting at 1 cell per well directly into 96-well plates. After approximately two to three weeks, single cell clones were expanded and subjected to genotyping. To identify complete genomic deletions caused by gRNA pairs, genomic PCR was performed with ...
Moreover, as sequencing depth increases, so too does the detection of cDNAs that likely correspond to spurious transcripts or genomic DNA contamination. Finally, even when dozens of barcoded individual cDNA libraries are sequenced in a single lane, the vast majority of transcripts in each sample ...