SUBSTANCE: method of DNA isolation from the human blood involves blood treatment with trimethyloctadecylammonium chloride solution, separation and solubilization of precipitate at high ionic strength following by DNA precipitation from solution with ethanol. Prepared preparation shows absence of RNA and ...
METHOD FOR THE ISOLATION OF DNA The subject invention concerns a novel process for the isolation of substantially pure DNA from biological samples. The process, advantageously, provides DNA of sufficient purity for subsequent use, as disclosed herein, without the neces... JACOB GRIMBERG,STANLEY NAWO...
However, preliminary validation of several STR systems for casework has demonstrated that the Chelex procedure is not the best method of DNA isolation for STR amplifications for our purposes. Long term storage at -20 degrees C in the presence of unbuffered Chelex beads (approximately 1 year), ...
SUBSTANCE: DNA from microorganisms is isolated by disrupting bacterial cells with lysing solution containing 80% of phenol, 18.8% of H<Sub>2</Sub>O and 0.5% of NaOH (pH 9.3-9.5). Method provides preparing preparation of bacterial DNA with high purity and nativity degree. Invention can be ...
In this paper, we describe a new modified method for extracting PCR-quality microbial community DNA from pig faecal samples, which combines the pretreatment with polyformaldehyde, and subsequent DNA lysis in the presence of CTAB, salt, PVP, and beta-mercaptoethanol, followed by isolation of nucleic...
DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species,...
Method for the isolation and purification of DNA molecules US4833239 * 1987年1月29日 1989年5月23日 Genetics Institute, Inc. Method for the isolation and purification of DNA moleculesUS4833239 * Jan 29, 1987 May 23, 1989 Genetics Institute, Inc. Method for the isolation and purification of.....
We have developed a simple and highly efficient protocol for isolating large quantities (150–400 μg/g leaf tissue) of high-quality DNA from fresh and frozenVitis vinifera leaves. Isolated DNA is essentially free of polysaccharides, polyphenols, and other major contaminants as judged by viscosity...
A general method of gene isolation has been developed that involves the chemical linkage of RNA to cellulose by a water-soluble carbodiimide, and the continuous circulation of DNA containing specific sequences complementary to the RNA. The temperature of the cellulose matrix is maintained at 37 degr...
摘要: A simple and rapid way for isolation of high quality genomic DNA from animal tissues was introduced in this paper. It was suggested that this method could be extensively applied in the experiments of molecular biology.关键词:genomic DNA animal tissue PCR RAPD ...