aDepletion of TSCMCD4 cells during aging. Freshly isolated PBMCs were collected and stained for flow cytometry. The statistical analysis was performed on unpaired samples (UMann–Whitney test) (*** forp < 0.0001).bRelationship between naive T-cell subsets during aging. The frequencies of TS...
naive CD4 T celldendritic cellallogeneic activationT-helper 1 cellsCD4 T cells, particularly those of the T-helper 1 (Th1) subset, are important effectors in alloimmune diseases, such as graft-versus-host disease, and in controlling infections with intracellular pathogens. Thus, it is plausible ...
Indirect activation of naive CD4þ T cells by dendritic cell-derived exosomes. Nat Immunol. 2002;3:1156–62. Article CAS PubMed Google Scholar Sarkar D, Ankrum JA, Teo GSL, Carman CV, Karp JM. Cellular and extracellular programming of cell fate through engineered intracrine-, paracrine-,...
目的探讨从一次性去白细胞滤器中回收naive CD4~+T cells,观察其增殖分化为CD4~+memory stem T cells的能力.方法分离一次性去白细胞滤器中的外周单个核细胞(PBMCs),并与直接从健康人外周血分离的PBMCs细胞成分进行比较;免疫磁珠分离法进一步纯化滤器中PBMCs的naive CD4~+T cells,观察其增殖与分化能力;分析诱导后的...
T cell preparation and treatment CD4+CD62L+ T cells were positively selected from murine splenocytes using a magnetic-activated cell-sorting system (Miltenyi Biotec, Bergisch Gladbach Germany). High purity naive T cells were plated into 12-well culture plates and cultured in Roswell Park Memoria...
et al. Long-lasting stem cell-like memory CD8+ T cells with a naive-like profile upon yellow fever vaccination. Sci. Transl. Med. 7, 282ra48 (2015). Article PubMed CAS Google Scholar Fuertes Marraco, S.A., Soneson, C., Delorenzi, M. & Speiser, D.E. Genome-wide RNA profiling...
Pancreatic β cells differentiated from stem cells provide promise for cell replacement therapy of diabetes. Human pluripotent stem cells could be differentiated into definitive endoderm, followed by pancreatic progenitors, and then subjected to endocrin
CD4+CD25+ cells were removed from the CD4+ T-cell pool, which showed more than 95% purity of CD4+CD25− T cells, using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotechnology, Germany) (Fig. 1a). The resulting CD4+CD25− T cells were cultured in the presence of ...
Mesenchymal stem cells generate a CD4+CD25+Foxp3+ regulatory T cell population during the differentiation process of Th1 and Th17 cells. Stem Cell Res Ther. 2013;4(3):65. Epub 2013/06/06. https://doi.org/10.1186/scrt216. Article CAS PubMed PubMed Central Google Scholar English K, ...
Cell and gene therapies using haematopoietic stem cells (HSCs) epitomize the transformative potential of regenerative medicine. Recent clinical successes for gene therapies involving autologous HSC transplantation (HSCT) demonstrate the potential of gene