We observed a significant fold increase of CD25+FOXP3+CD127- T cells within CD4+ T cell co-cultures with CD56bright NKTGF-β in comparison to stimulated T cells alone (Figure 2I), indicating CD56bright NKTGF-β could promote naive CD4+ T cells differentiation into regulatory T cells (...
Adipose derived mesenchymal stem cell exosomes loaded with miR-10a promote the differentiation of Th17 and Treg from naive CD4 + T cellMesenchymal stem cellsmiRNAsT cells differentiationExosomesAims The balance between various CD4 T cell subsets through highly regulated differentiation of nave T cells ...
The filtered single cell suspension was lysed by adding erythrocyte lysate (Solarbio) for 3 min, then centrifuged to remove the supernatant and washed with PBS to obtain a clean cell suspension. The desired CD4+ T cells were sorted using the stem cell isolation kit (Cat#19852A; StemCell ...
Stem Cell 13058 StemSep Mu-Hu Chimera Depletion Kit ￥8,728.00 Stem Cell 13060 Anti-Biotin Tetramer, 10 mL ￥33,578.00 Stem Cell 13061 StemSep Mouse T Cell Kit, 5x10e9 cells ￥37,920.00 Stem Cell 13062 StemSep Mouse CD4+ T Cell Kit, 5x10e9 ￥37,920.00 Stem Cell 13063 StemSep Mouse...
other CD4 T-cell subsets (Fig.3c, left panel and Supplementary Fig.2A, B). TCD31negNAIVE, TRTE, TMNP, and TSCMCD4 T cells exhibited distinctive gene expression profiles (Fig.3c, right panel), while non-RTE naive and TMNPclustered closely; the ontogenic proximity of the latter subsets ...
3d). Neither CD4+ T cell population proliferated significantly in naive mice, suggesting that TCR 1 does not recognize the wild-type CLTC epitope in vivo despite producing IFN-γ in response to splenocytes pulsed with high concentrations of the corresponding peptide (Figs. 3d and 1f). ...
Innate lymphoid cells have the capability to communicate with other immune cell types to coordinate the immune system functioning during homeostasis and inflammation. However, these cells behave diff...
CD4+CD25+ cells were removed from the CD4+ T-cell pool, which showed more than 95% purity of CD4+CD25− T cells, using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotechnology, Germany) (Fig. 1a). The resulting CD4+CD25− T cells were cultured in the presence of ...
Regulation of T-cell apoptosis is an important mechanism controlling the number of activated T cells. We therefore examined apoptosis by Annexin V staining on ex vivo EdU (5- ethynyl-2'-deoxyuridine)-incorporated CD4- and CD8-sorted T cells from untreated, irradiated, and irradiated-MSC-treated...
Mehta-Damani et al., “Generation of antigen-specific CD4+ T cell lines from naïve precursors,” Eur. J. Immunol., 5:1206-11 (1995). Mehta-Damani et al., “Generation of antigen-specific CD8+ CTLs from naive precursors,” J. Immunol., 153:996-1003 (1994). Melcher et al., ...