Stem Cell 13058 StemSep Mu-Hu Chimera Depletion Kit ¥8,728.00 Stem Cell 13060 Anti-Biotin Tetramer, 10 mL ¥33,578.00 Stem Cell 13061 StemSep Mouse T Cell Kit, 5x10e9 cells ¥37,920.00 Stem Cell 13062 StemSep Mouse CD4+ T Cell Kit, 5x10e9 ¥37,920.00 Stem Cell 13063 StemSep Mouse...
目的探讨从一次性去白细胞滤器中回收naive CD4~+T cells,观察其增殖分化为CD4~+memory stem T cells的能力.方法分离一次性去白细胞滤器中的外周单个核细胞(PBMCs),并与直接从健康人外周血分离的PBMCs细胞成分进行比较;免疫磁珠分离法进一步纯化滤器中PBMCs的naive CD4~+T cells,观察其增殖与分化能力;分析诱导后的...
Naive CD4+ T cells were labeled with CFSE (5 µM, 5 min) and differentiated into Th1 and Th17 cells under MSC-sEVs treatment conditions. sEVs specifically suppressed the differentiation of Th17 cells, and their mechanisms were independent of cell cycle arrest. A representative image from...
Despite an abundance of literature on the differentiation of CD4 T cells, the ontogeny of naive or early-stage memory CD4 T-cell subsets is poorly understood. Studies generally fail to appreciate their heterogeneity by grouping CD45RO−CCR7+CD27+CD62L+CD4 T cells into a homogeneous TNAIVEc...
A Schematic protocol for isolation CD4+ T cells and co-culturing with MSCs or IL-10-MSCs. B–C Th2 cell levels after co-culturing with MSCs or IL-10-MSCs (n = 12). D–E IL-13 and IL-5 levels in supernatants were analysed using ELISA. Data are shown as mean ± SEM. ...
cytokines IL-17 and IL-22, and increase Treg-related IL-10 expression in both lung and plasma [44] In vitro coculture study showed mouse BM-MSCs inhibited the differentiation of Th17 cells from naive CD4+ T cells via the programmed death-1 (PD-1) pathway through cell-to-cell contact [...
CD4+CD25+ cells were removed from the CD4+ T-cell pool, which showed more than 95% purity of CD4+CD25− T cells, using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotechnology, Germany) (Fig. 1a). The resulting CD4+CD25− T cells were cultured in the presence of ...
These CD4+ T cells showed intracellular IFN-γ production when they were restimulated with CMV DC, whereas CD8+ T cells or control CD4+ T cells did not express IFN-γ (Fig. 4). Table 1. Immunophenotypes and killing activities of cultured lymphocytes from various cell sources Empty Cell ...
(PBMCs) from buffy coat or heparinized blood obtained from the blood bank (UNN, Tromsø Norway), according to the manufacturer’s instructions. Enriched CD4+ T cells were obtained by negative selection from lymphoprep-PBMCs pellets using the magnetic CD4+ T cell human isolation kit (130-096...
Results Induction of naive-like T cells from activated CD4 þ T cells. The GSK-3b inhibitor TWS119 has been used for TSCM cell generation from naive T cells by arresting effector T cell differ- entiation13,14. This method successfully worked for naive CD8 þ T cells, but it was not...