Karyotyping was normal and doubling time of clonal cell growth was estimated to be 70.7 ± 14.5 h (mean ± standard deviation). The surface phenotype of the cells was positive for CD73, CD166, CD29, CD49a, CD49b, CD49d, CD44, CD105, and MHC class I, and negative for CD34, CD...
Interestingly, the CD4-population had significant life-sparing activity, even though this population was depleted of long-term repopulating stem cells when compared with CD4+ cells. The majority of the cells that respond to the stroma in Whitlock-Witte cultures with B-cell differentiation were ...
IDO activity of both cell types was measured after stimulation with IFNγ by a colorimetric assay. MenSCs exhibited dual mesenchymal and embryonic markers and multi-lineage differentiation capacity. MenSCs significantly increased proliferation of CD4+ cells at ratios 1:2, 1:4 and 1:8. IFNγ pre...
Bone marrow is rich in hematopoietic stem cell content in the isolation of MSC. However, the difficulties in the collection procedure and the need for general anesthesia have restricted the use of bone marrow in both clinical and research studies [6]. Perinatal sources such as the placenta, umb...
Methods of MSC-Exos isolation MSC-Exos to be used in clinical trials must be produced under the GMP-grade condition in which the cell culture environment, cultivation system, dissipation enzyme, and culture medium are strictly monitored using GMP standards. MSC-Exos need to be purified and charac...
Article Open access 30 September 2024 Cancer stem cells: advances in knowledge and implications for cancer therapy Article Open access 05 July 2024 Tumor cell plasticity in targeted therapy-induced resistance: mechanisms and new strategies Article Open access 11 March 2023 Introduction...
T Cell Isolation LymphoprepTM(STEMCELL Technologies) was used to isolate peripheral blood mononuclear cells (PBMCs) from buffy coat or heparinized blood obtained from the blood bank (UNN, Tromsø Norway), according to the manufacturer’s instructions. Enriched CD4+ T cells were obtained by negativ...
The T cell inhibition by MSC was not due to the soluble HLA-G5 isoform, but to the surface expression of HLA-G1, as shown by the need of cell-cell contact and by the use of neutralizing anti-HLA-G antibodies. To note, in a HLAG- mediated fashion, MSC facilitated the expansion of...
These findings prompted us to further probe the efficacy of CD4+ T cells expressing either TCR 1 or TCR 3. As antitumor efficacy was dependent on CD40L help for CD8+ T cells, we used the SCC VII parental cell line for all future experiments to rule out the contribution of CD8+ T cells...
Mesenchymal stromal/stem cells (MSCs) are widely utilized in cell therapy because of their robust immunomodulatory and regenerative properties. Their paracrine activity is one of the most important features that contribute to their efficacy. Recently, it has been demonstrated that the production of vari...