Interestingly, the CD4-population had significant life-sparing activity, even though this population was depleted of long-term repopulating stem cells when compared with CD4+ cells. The majority of the cells that respond to the stroma in Whitlock-Witte cultures with B-cell differentiation were ...
CD4+CD25+ cells were removed from the CD4+ T-cell pool, which showed more than 95% purity of CD4+CD25− T cells, using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotechnology, Germany) (Fig. 1a). The resulting CD4+CD25− T cells were cultured in the presence of ...
Mesenchymal stromal/stem cells (MSCs) are widely utilized in cell therapy because of their robust immunomodulatory and regenerative properties. Their paracrine activity is one of the most important features that contribute to their efficacy. Recently, it has been demonstrated that the production of vari...
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These findings prompted us to further probe the efficacy of CD4+ T cells expressing either TCR 1 or TCR 3. As antitumor efficacy was dependent on CD40L help for CD8+ T cells, we used the SCC VII parental cell line for all future experiments to rule out the contribution of CD8+ T cells...
The diversity of the naïve T cell repertoire drives the replenishment potential and capacity of memory T cells to respond to immune challenges. Attrition of the immune system is associated with an increased prevalence of pathologies in aged individual
Resident adult epithelial stem cells maintain tissue homeostasis by balancing self-renewal and differentiation. The stem cell potential of human epidermal keratinocytes is retained in vitro but lost over time suggesting extrinsic and intrinsic regulation
The cells that express VEGFR-1 or the marker bind to the molecule, permitting separation and isolation of the cells of interest. If the cells do not internalize the molecule or marker, it can be separated from the cell by methods known in the art. For example, antibodies can be separated...
RECENT FINDINGS: Among CD4+ T cells, the best described are the naturally occurring CD4+CD25+ regulatory T cells and type 1 regulatory T cells. Recent progress has been made in the characterization of both subsets in terms of isolation and induction, respectively. However, a clear definition ...
T cell potential is positively identified by the presence of a CD4+CD8+ population following 21+ days of OP9-DL4 coculture, while an absence of potential is identified by an absence of CD45+ lymphocytes3,30. C, Heatmaps visualizing the mean expression of HOXA genes across all biological ...