MAGM208 MagCellect Mouse CD4+ CD25+ Regulatory T Cell Isolation Kit (1 KT)MAGM209 MagCellect Mouse Hemaoietic Cell Lineage Depletion Kit (1 KT)MAGM210 MagCellect Mouse NK Cell Isolation Kit (1 KT)MAGM212 MagCellect Mouse Mesenchymal Stem Cell Isolation Kit (1 KT)MAGR301 MagCellect Rat...
On day 3 after immunization, single-cell suspensions were obtained from LNs of C57/Bl6 wt mice. CD4+ T cells were isolated using the mouse CD4+ T cell isolation kit (Stem Cell Technologies, Vancouver, BC, Canada), according to the manufacturer's protocol. The lymphocytes were fluorescently ...
Sources and preparation of antibodies specific for the mouse cell surface antigens CD4 (GK1.5), Gr-1 (Raw-8C5), B220 (Ra3-6B2), Mac-l(Ml/70), CD5 (53.7.3), CD8 (53.6.7), and Thy-1 (31–11) have been described previously.16 Second stage reagent included goat anti-rat ...
The 58α−β− cells expressing TCRs 1 and 2 were able to bind tetramer independently of the CD4 co-receptor, whereas cells expressing TCRs 3 and 4 did not, despite comparable levels of TCR expression (Fig. 2e). In conclusion, the CLTCH129>Q-specific CD4+ T cell pool contains T ...
The diversity of the naïve T cell repertoire drives the replenishment potential and capacity of memory T cells to respond to immune challenges. Attrition of the immune system is associated with an increased prevalence of pathologies in aged individual
while enhancing Nrf2 activity was proven to effectively counteract cellular senescence and render them similar to young cell [178,213]. Exosomes derived from MSCs were observed to significantly alleviate aging-related senescence of CD4 + T cells. This reduction in senescence was attributed to the...
These CD4+ T cells showed intracellular IFN-γ production when they were restimulated with CMV DC, whereas CD8+ T cells or control CD4+ T cells did not express IFN-γ (Fig. 4). Table 1. Immunophenotypes and killing activities of cultured lymphocytes from various cell sources Empty Cell ...
Exosomes derived from mesenchymal stem cells primed with disease-condition-serum improved therapeutic efficacy in a mouse rheumatoid arthritis model via enhanced TGF-β1 production Eun Wha Choi, I.-Rang Lim, Ji Hong Park, Jiwoo Song, Bongkum Choi & Sungjoo Kim Stem Cell Research & Therapy vol...
In the current study, we aimed to investigate the effect of extracellular vesicles (EVs) derived from human umbilical cord-MSC (hUCSC-EV) on the clinical score and inflammatory/anti-inflammatory cytokines on the EAE mouse model. After induction of EAE in C57Bl/6 mice, they were treated ...
Fig. 3: ID1-expressing TAMs promote CRC progression partially through inhibiting CD8+T cell recruitment. aTumor inhibition rate of MC38 and TAMs mixture s.c. model in the indicated groups,n = 8 mice per group, Welch’s test.b,cPercentage of CD8+T cells (b), representative mIHC ima...